Fig 1.
Phylogenetic tree of MIOX proteins from 8 Gossypium species, Gossypioides kirkii and Arabidopsis thaliana.
The different-colored arcs indicate different groups of MIOX proteins. The green circles and red boxes represent MIOX proteins from A genome and D genome, respectively, black triangles represent MIOX proteins from Gossypioides kirkii, blue stars represent MIOX proteins from Arabidopsis thaliana.
Fig 2.
The exon–intron structures and conserved motifs of MIOX.
The length of proteins and DNA sequence was estimated using the scale at the bottom, black line indicated the non-conserved amino acid or introns. (a) The conserved motifs of MIOX gene family; (b) The gene structure of MIOX gene family.
Fig 3.
Distribution of the MIOX genes on the cotton chromosomes.
The vertical bar located on the left side indicates the chromosome sizes in mega bases (Mb), the chromosome number is located above each chromosome.
Fig 4.
Collinearity of MIOX genes between different cotton species.
(a) Collinearity between A-genome of G. arboretum and A-subgenome of G. hirsutum and G. barbadense; (b) Collinearity between D-genome of G. raimondii and D-subgenome of G. hirsutum and G. barbadense.
Fig 5.
The Ka/Ks ratio of MIOX orthologous gene pairs of each group.
Fig 6.
Number of MIOX gene cis-elements in each group.
Fig 7.
Expression profiles of MIOX genes in different tissues and during different developmental stages of ovule and fiber.
Fig 8.
Expression profiles of the MIOX genes in response to different stresses.
Fig 9.
Expression of MIOX genes under different stress conditions.
Table 1.
The specific primers for qRT-PCR of G. hirsutum MIOX genes.