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Antidiabetic properties of Dioscoreophyllum cumminsii (Stapf) Diels stem bark on streptozotocin-induced diabetic rats

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Abstract

Traditionally, Dioscoreophyllum cumminsii has been reported for its ability to treat diabetes; however, the stem bark of the plant has not been scientifically exploited for its antidiabetic activity. The study aims to investigate the effect of the antidiabetic properties of Dioscoreophyllum cumminsii stem extract on streptozotocin-induced diabetic rats. Thirty rats were made diabetic by a single intraperitoneal administration of streptozocin (STZ), at a dose of 45 mg/kg bw. After confirming they are diabetic, 21 days of administration was conducted on the rats which are divided into 6 groups; Control (not diabetic group); 100 mg/kg bw Metformin (standard group), Untreated group, and then 50 mg/kg bw, 100 mg/kg bw and 200 mg/kg bw of Dioscoreophyllum cumminsii stem extract. Analyses were carried out on the serum and liver after it was excised from the animals. The activity of liver G6PD and serum HDL was increased significantly in the group treated with metformin and 200 mg/kg bw extract. A significant decrease was observed in the liver glycogen concentration in the 50 mg/kg bw group. The liver glucose concentration showed a significant increase in the groups treated with 50, 100, and 200 mg/kg bw plus the metformin group. Also, there was a significant increase in triglyceride concentration in 50 mg/kg bw of the extract. The findings revealed that in a dose-dependent manner, the aqueous extract of D. cumminsii stem bark demonstrated hypoglycemic potential compared to the standard drug, metformin, thus, indicating the antidiabetic properties of Dioscoreophyllum cumminsii stem extract on streptozotocin-induced diabetic rats.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  • Abd El-Hameed AM, Yousef AI, Abd El-Twab SM, El-Shahawy AA, Abdel-Moneim A (2021) Hepatoprotective effects of polydatin-loaded chitosan nanoparticles in diabetic rats: modulation of glucose metabolism, oxidative stress, and inflammation biomarkers. Biochem Mosc 86:179–189

    Article  CAS  Google Scholar 

  • Abiodun OA, Akinoso R (2014) Physico-chemical properties of Serendipity berry (Dioscoreophyllum cumminsii) fruit. J Appl Sci Environ Manag 18(2):218–221

    Google Scholar 

  • Afrisham R, Aberomand M, Ghaffari MA, Siahpoosh A, Jamalan M (2015) Inhibitory effect of Heracleum persicum and Ziziphus jujuba on activity of alpha-amylase. J Botany

  • Albers JJ, Warmick GR, Cheng MC (1978) Determination of high-density lipoprotein (HDL)–cholesterol. Lipids 13:926–932

    Article  CAS  PubMed  Google Scholar 

  • Balogun EA, Ayeni SD, Otolorin AAO, Rotimi DE (2021) Anti-diabetic and antidyslipidemic activities of Entandrophargma cylindricum extract on streptozotocin-induced diabetic rats. Biointerface Res Appl Chem 11(6):14251–14259

    Article  CAS  Google Scholar 

  • Baynes HW (2015) Classification, pathophysiology, diagnosis and management of diabetes mellitus. J Diabetes Metab 6(5):1–9

    Google Scholar 

  • Bernfeld P (1955) Methods in enzymology; Colowick, SP, Kaplan, NO, Eds. Academic Press New York. 1:149

  • Blahova J, Martiniakova M, Babikova M, Kovacova V, Mondockova V, Omelka R (2021) Pharmaceutical drugs and natural therapeutic products for the treatment of type 2 diabetes mellitus. Pharmaceu 14(8):806

    Article  CAS  Google Scholar 

  • Brandstrup N, Kirk JE, Bruni C (1957) The hexokinase and phosphoglucoisomerase activities of aortic and pulmonary artery tissue in individuals of various ages. J Gerontology 12(2):166–171

    Article  CAS  Google Scholar 

  • Ezuruike UF, Prieto JM (2014) The use of plants in the traditional management of diabetes in Nigeria: pharmacological and toxicological considerations. J Ethnopharma 155(2):857–924

    Article  CAS  Google Scholar 

  • Fredrickson DS, Levy RL, Lees RS (1967) Monoreagent enzymatic cholesterol. New Engl J Med 276:148–156

    Article  CAS  PubMed  Google Scholar 

  • Gancedo JM, Gancedo C (1971) Fructose-1, 6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and non fermenting yeasts. Arch Mikrobiol 76(2):132–138

    Article  CAS  PubMed  Google Scholar 

  • Giacco R, Costabile G, Della Pepa G, Anniballi G, Griffo E, Mangione A, Cipriano P, Viscovo D, Clemente G, Landberg R, Pacini G (2014) A whole-grain cereal-based diet lowers postprandial plasma insulin and triglyceride levels in individuals with metabolic syndrome. Nutr Metab Cardiovasc Dis 24(8):837–844

    Article  CAS  PubMed  Google Scholar 

  • Ibitoye OB, Ghali UM, Adekunle JB, Uwazie JN, Ajiboye TO (2017) Antidyslipidemic, anti-inflammatory, and antioxidant activities of aqueous leaf extract of Dioscoreophyllum cumminsii (Stapf) Diels in high-fat diet-fed rats. Evid-Based Complementary Altern Med

  • Karau GM, Njagi EN, Machocho AK, Wangai LN, Kamau PN (2014) Efficacy and safety assessment of Launaea cornuta extracts in the management of diabetes mellitus. Ame J Med Med Sci 4(1):1–7

    Google Scholar 

  • Kayode OT, Rotimi DE, Olaolu TD, Adeyemi OS (2020) Ketogenic diet improves and restores redox status and biochemical indices in monosodium glutamate-induced rat testicular toxicity. Biomed & Pharma 1(127):110227

    Article  Google Scholar 

  • Koide H, Oda T (1959) Pathological occurrence of glucose-6-phosphatase in serum in liver diseases. Clin Chim Acta 4:554–561

    Article  CAS  PubMed  Google Scholar 

  • Kooti W, Farokhipour M, Asadzadeh Z, Ashtary-Larky D, Asadi-Samani M (2016) The role of medicinal plants in the treatment of diabetes: a systematic review. Elect Physician 8(1):1832

    Article  Google Scholar 

  • Lei P, Chen H, Ma J, Fang Y, Qu L, Yang Q, Sun D (2022) Research progress on extraction technology and biomedical function of natural sugar substitutes. Front Nutr 9:952147

  • Levesque C (2017) Therapeutic lifestyle changes for diabetes mellitus. Nurs Clin 52(4):679–692

    Google Scholar 

  • Mukesh R, Namita P (2013) Medicinal plants with antidiabetic potential-a review. American-Eurasian J Agric Environ Sci 13(1):81–94

    Google Scholar 

  • Mukhtar Y, Galalain A, Yunusa U (2020) A modern overview on diabetes mellitus: a chronic endocrine disorder. European Journal of Biology 5(2):1–14

    Article  Google Scholar 

  • Ojo OA, Okesola MA, Ekakitie LI, Ajiboye BO, Oyinloye BE, Agboinghale PE, & Onikanni AS (2020). Gongronema latifolium Benth. leaf extract attenuates diabetes‐induced neuropathy via inhibition of cognitive, oxidative stress and inflammatory response. J Sci Food Agric 100(12):4504–4511

  • Ojo OA, Amanze JC, Oni AI, Grant S, Iyobhebhe M, Elebiyo TC, Ojo AB (2022a) Antidiabetic activity of avocado seeds (Persea americana Mill.) in diabetic rats via activation of PI3K/AKT signaling pathway. Sci Rep 12(1):2919

  • Ojo OA, Grant S, Amanze JC, Oni AI, Ojo AB, Elebiyo TC, Ogunlakin AD (2022b) Annona muricata L. peel extract inhibits carbohydrate metabolizing enzymes and reduces pancreatic β-cells, inflammation, and apoptosis via upregulation of PI3K/AKT genes. Plos One 17(10):e0276984

  • Olaolu DT, Rotimi DE, Olaolu AP (2018) Effect of alcohol infusion of Cissus populnea root on testicular function and serum hormone of male Wistar rats. Asian Pac J Reprod 7(3):117–22

  • Oloyede HO, Bello TO, Ajiboye TO, Salawu MO (2015) Antidiabetic and antidyslipidemic activities of aqueous leaf extract of Dioscoreophyllum cumminsii (Stapf) Diels in alloxan-induced diabetic rats. J Ethnopharm 166:313–322

    Article  CAS  Google Scholar 

  • Ozougwu JC, Obimba KC, Belonwu CD, Unakalamba CB (2013) The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. J Physio Pathophysio4(4):46–57

  • Padhi S, Nayak AK, Behera A (2020) Type II diabetes mellitus: a review on recent drug based therapeutics. Biomedicine & Pharmacother 131:110708

    Article  CAS  Google Scholar 

  • Passonneau JV, Lauderdale VR (1974) A comparison of three methods of glycogen measurement in tissues. Analy Biochem 60(2):405–412

    Article  CAS  Google Scholar 

  • Sofowora A (1993) Medicinal plants and traditional medicine in Africa. John Wiley & Sons, Nigeria, p 256

    Google Scholar 

  • Sulyman AO, Akolade JO, Sabiu SA, Aladodo RA, Muritala HF (2016) Antidiabetic potentials of ethanolic extract of Aristolochia ringens (Vahl.) roots. J Ethnopharma 182:122–128

    Article  CAS  Google Scholar 

  • Trease GE, Evans WC (2002) Pharmacognosy. 15th Ed. London: Saunders Publishers. p 221–229. ISBN 8131200876

  • Yanai H, Yoshida H (2019) Beneficial effects of adiponectin on glucose and lipid metabolism and atherosclerotic progression: mechanisms and perspectives. Int J Mol Sci 20(5):1190

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zheng Y, Ley SH, Hu FB (2018) Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol 14(2):88

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Ilorin, Kwara State, Ilorin, Nigeria

    Elizabeth A. Balogun, Al-Ameen O. Otolorin & Samuel D. Ayeni

  2. Department of Biochemistry, Landmark University, Kwara State, Omu Aran, Nigeria

    Damilare E. Rotimi

  3. Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Osun State, Iwo, Nigeria

    Oluwafemi Adeleke Ojo

Authors
  1. Elizabeth A. Balogun
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  2. Al-Ameen O. Otolorin
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  3. Samuel D. Ayeni
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  4. Damilare E. Rotimi
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  5. Oluwafemi Adeleke Ojo
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Contributions

EAB and AOO contributed to the design of the study. AOO carried out the analysis. AOO and SDA participated in data collection and statistical analysis. AOO, DER, and OAO wrote the first draft of the manuscript. EAB, AOO, SDA, DER, and OAO perused and approved the final manuscript.

Corresponding authors

Correspondence to Damilare E. Rotimi or Oluwafemi Adeleke Ojo.

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This study was not supported by any funding.

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The authors declare that they have no conflict of interest.

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The animals were handled following the institution’s Animal Ethics Committee in compliance with the Guidelines for the Use and Care of Lab. Animals and the study were given their approval (approval number: UERC/ASN/2015/118) and reported in accordance with Animal Research Reporting of in vivo Experiments (ARRIVE) guidelines.

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Balogun, E.A., Otolorin, AA.O., Ayeni, S.D. et al. Antidiabetic properties of Dioscoreophyllum cumminsii (Stapf) Diels stem bark on streptozotocin-induced diabetic rats. Comp Clin Pathol 32, 751–759 (2023). https://doi.org/10.1007/s00580-023-03482-1

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