Abstract
A new species, Barleria lavaniana (Acanthaceae), is described and illustrated. It resembles B. longiflora but differs from it in the characters of calyx, stamen, style and stigma. Stem anatomy reveals that vessel elements are mostly angular with a short tail at one end and a long tail on the other end. Xylem rays are usually biseriate and xylem fibres relatively thin-walled. Interxylary phloem is generally characterized by a single companion cell. The somatic chromosome number was 2n = 2x = 40. The karyotype is moderately symmetrical and fell in Stebbins’ 3b category. Chromosomes have median and submedian region centromeres. Based on cpDNA data, the new species is placed in subgenus Barleria and shows phylogenetic affinity towards B. longiflora and B. acuminata. Our findings suggest the need for comprehensive phylogenetic analyses of the genus Barleria based on high-throughput sequencing data to understand species interrelationships and biogeography.
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Acknowledgements
Authors thank the Head, Department of Botany, Shivaji University, for providing necessary facilities. We are grateful to Dr. Kishore S. Rajput, Associate Professor, Department of Botany, The Maharaja Sayajirao University of Baroda, Vadodra, Gujarat 390002, for helping us with stem anatomy studies. SSP thanks Ministry of Environment, Forest and Climate Change, Govt. of India, for providing Junior Research Fellowship vide sanction letter F.No.10/04/2012-CS/BG dated 19/11/2013. AST thanks Council of Scientific and Industrial Research, New Delhi, for Senior Research Fellowship (SRF) (Award Number–09/816(0042)/2018-EMR-I and ACK No–113307/2K17/1). We are grateful to Dr. Shrikant P. Sutar, Department of Botany, Nowrosjee Wadia College, Maharashtra for the illustrations. We thank the reviewers for the critical comments that improved the content of the manuscript.
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Appendices
Appendix 1
Voucher information of Barleria longiflora.
India, s.d., s.coll., s.n. (E00892701, E00892710, E00892711) (E); s.d., J.G. Konig s.n. (10004760, 10004762, 10004763) (C); s.d., K. Narayana Iyer s.n. (Acc. No. 01899) (TBGT); s.d., W. Roxburgh s.n. (E00874943, E00892702); s.d., P. Russell s.n. (E00874942); 1961, Wight s.n. (E00892703, E00892704, E00892712) (E); 1961, Wight 2501c (E00892708) (E). Andhra Pradesh, Guntur district, Mellavagu, 3 Sep 1956, S.K. Wagh 3619, 3620, 3622 (BLAT), Nagarjunanagar, 13 Dec 1959, Sebastine 9751, Nagarjunakonda valley, 27 Nov 1961, K. Thothathri 9807 (CAL); Krishna district, Kondahalli, Jan 1887, J.S. Gamble 18576; Kundapalli, 25 Aug 1956, S.K. Wagh 3290, 3291 (BLAT); Kurnool district, Rangapurum R. F., 11 Oct 1983, J.S. Gamble 1706; Prakasam District, Gangavaram hill, 3 Dec 1983, R.K. Mohan 0319; Orvakal mountains, 8 May 2004, S.K. Nageervddin 622 (CAL). Karnataka, Raichur district, Bankaldoddi R.F., Deodurg, 14 Nov 1975, N.P. Singh 141692 (BSI). Kerala, Palakkad district, Attappadi, 12 Dec 1973, J.G. Menon 103 (TBGT). Tamil Nadu, Dharmapuri district, Dharmapuri, 7 Dec 1978, K.M. Mathew 19714 (CAL); Dindigul district, Sirumalai hills, 26 May 1958, J. Pallithanam 3462, 5 Feb 1959, J. Pallithanam 4288 (BLAT); Kanchipuram district, Pallavaram, s.d., s.coll., s.n. (E00892705) (E); Thirukazhukundram, s.d., K. Narayana Iyer s.n. (Acc. No. 01552) (TBGT); Vandalur hills, 10 Jan 1958, H. Santapau 22344 (BLAT); Kanayakumari district, Gomuki-Vellimalai, 7 Feb 1983, K. Ramamurthy 77365 (CAL); Nagapattinam district, Nagapattinam, s.d., Wight s.n. (E00892714) (E); Vandalur, 10 Jan 1958, K. Subramanyam 5413; Vandalur R. F., 3 Feb 1976, A.N. Henry 47153; North Arcot district, Sathanur dam forest, 26 Nov 1971, Vajravelu 52091 (CAL); Puducherry district, Pondicherry, 1834, M. Ferrollet s.n. (E00892706) (E); South Arcot district, Ginjee R.F., 17 Mar 1961, Sebastine 12171; Ginjee to Chokuptha, 18 Feb 1889, K. Ramamurthy 60308; Gomukhi dam, Thagari forest north, 19 Jan 1978, Ramamurthy 52852; 14 Dec 1978, Matthew and Perumal 20215 (CAL); Travancore, 1817, s.coll., 2501a (K001116222), 2501b (K001116224), 2501c (K001116223) (K); 1817, s.coll., 2501a (E00892707) (E); Tiruchirapalli district, Kolli Hills, Puliancholai, 30 Nov 1975, Anobaran 19570; Tirunelveli District, Kalkad hill, 7 Feb, C.E.C. Fischer 3873 (CAL).
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Online Resource 1. Phylogenetic analyses using maximum likelihood and maximum parsimony method.
Online Resource 2. Aligned matrix of rbcL + matK dataset.
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Patil, S.S., Tamboli, A.S., Yadav, S.R. et al. A new species of Barleria (Acanthaceae), its morphotaxonomy, cytogenetics and phylogenetic placement. Plant Syst Evol 305, 933–947 (2019). https://doi.org/10.1007/s00606-019-01613-2
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DOI: https://doi.org/10.1007/s00606-019-01613-2