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Cross-species transferability of EST-SSR markers derived from the transcriptome of kenaf (Hibiscus cannabinus L.) and their application to genus Hibiscus

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Abstract

The genus Hibiscus comprises approximately 300 diverse species whose genetic relationships have not been extensively clarified. In this study, we evaluated the cross-species transferability of 102 expressed sequence tag-simple sequence repeat (EST-SSR) markers derived from the transcriptome of kenaf H. cannabinus L. and used them to assess the genetic diversity and inter-relationships of 87 accessions and 7 cultivars of 18 Hibiscus species. Among 102 markers, 100 (98%) were polymorphic in these 94 accessions/cultivars. The rates of cross-species transferability of the EST-SSR markers ranged from 83 H. trionum L. to 99% H. ponticus Rupr., with an average of 89.9%. A total of 871 alleles were detected using the 100 polymorphic EST-SSR markers; the number of alleles ranged from 2 (RBRC_Hc_ES_76) to 16 (RBRC_Hc_ES_80) and averaged 8.71. Polymorphism information content values varied from 0.15 (RBRC_Hc_ES_74) to 0.84 (RBRC_Hc_ES_3, RBRC_Hc_ES_95), with an average of 0.56. Phylogenetic and population structure analyses classified the 94 accessions/cultivars into two and two or three groups, respectively. Overall, our results demonstrate that the EST-SSR markers derived from the kenaf transcriptome were applied successfully to other Hibiscus species to provide useful information on the genetic diversity of this genus.

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Acknowledgements

This work was supported by the Nuclear R&D program of KAERI and Radiation Technology R&D Program (NRF-2017M2A2A6A05018538) through the National Research Foundation of Korea funded by the Ministry of Science and ICT. We thank Margaret Biswas, Ph.D., from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.

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  1. Jung Min Kim and Jae Il Lyu have contributed equally to this work.

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  1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Korea

    Jung Min Kim, Min-Kyu Lee, Dong-Gun Kim, Jin-Baek Kim, Joon-Woo Ahn & Soon-Jae Kwon

  2. Division of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, Korea

    Jung Min Kim, Jae Il Lyu, Min-Kyu Lee & Bo-Keun Ha

  3. Department of Life-Resources, Graduate School, Sunchon National University, Sunchŏn, 59722, Korea

    Dong-Gun Kim

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10722_2019_817_MOESM1_ESM.pptx

Supplementary Fig. S1. EST-SSR patterns showing polymorphism and transferability of 18 Hibiscus species based on the RBRC_Hc_ES_4, RBRC_Hc_ES_20, and RBRC_Hc_ES_79 loci (PPTX 319 kb)

Supplementary Fig. S2. Graphical determination of the optimal number of populations (K) (PPTX 127 kb)

Supplementary Table S1. Polymorphism analysis of 101 EST-SSR markers in 18 Hibiscus species (DOCX 30 kb)

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Kim, J.M., Lyu, J.I., Lee, MK. et al. Cross-species transferability of EST-SSR markers derived from the transcriptome of kenaf (Hibiscus cannabinus L.) and their application to genus Hibiscus. Genet Resour Crop Evol 66, 1543–1556 (2019). https://doi.org/10.1007/s10722-019-00817-2

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