Skip to main content

Advertisement

SpringerLink
Log in

Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Endophytic fungal interaction with medicinal plant resulting in the production of bioactive compounds influenced the present study. Endophytic fungus Penicillium pinophilum Hedgc. from Alloteropsis cimicina with high incidence was isolated by incubation methods and characterized by morphological and molecular (ITS rDNA region) methods. Penicillium pinophilum was cultured on PD broth and metabolites of host and endophyte were extracted with ethyl acetate and methanol. Metabolites were assayed for antimicrobial potential by well diffusion and scavenging ability by spectrophotometric and electrochemical methods. Metabolite profiling by Orbitrap High-Resolution Liquid Chromatography-Mass Spectrometry and Fourier-transform infrared spectroscopy and by validation of antimicrobial and antioxidant activities in-silico drug-likeness of spectral compound prediction and molecular docking were performed. Host and P. pinophilum extracts showed strong antimicrobial potential against certain clinical bacterial strains and Fusarium oxysporum. Fungal extracts exhibited higher antioxidant activity than A. cimicina extract. Metabolite profiling indicated 14 and 21 antimicrobial, 10 and 13 antioxidant compounds in A. cimicina and P. pinophilum extracts, respectively. There were eight spectral compounds common to endophyte and host with high binding affinity towards receptors. The present study revealed that P. pinophilum and A. cimicina are natural reservoirs of novel bioactive compounds with antimicrobial and antioxidant properties.

Graphic Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data availability

Data will be available online after the acceptance of the manuscript in http://www.splink.org.

References

  1. Dwibedi V, Saxena S (2020) In vitro anti-oxidant, anti-fungal and anti-staphylococcal activity of resveratrol-producing endophytic fungi. Proc Natl Acad Sci USA 90(1):207–219

    CAS  Google Scholar 

  2. Kusari S, Hertweck C, Spiteller M (2012) Chemical ecology of endophytic fungi: origins of secondary metabolites. Chem Bio 19(7):792–798

    Article  CAS  Google Scholar 

  3. Shweta S, Zuehlke S, Ramesha BT, Priti V, Kumar PM, Ravikanth G, Spiteller M, Vasudeva R, Shaanker RU (2010) Endophytic fungal strains of Fusarium solani, from Apodytes dimidiata E. Mey ex Arn (Icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin. Phytochemistry 71(1):117–122

    Article  CAS  Google Scholar 

  4. Sette LD, Passarini MRZ, Delarmelina C, Salati F, Duarte MCT (2006) Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World J Microb Biot 22(11):1185–1195

    Article  CAS  Google Scholar 

  5. Qin S, Li J, Chen HH, Zhao GZ, Zhu WY, Jiang CL, Xu LH, Li WJ (2009) Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna. China App Environ Microbial 75(19):6176–6186

    Article  CAS  Google Scholar 

  6. Katewa SS, Guria BD, Jain A (2001) Ethnomedicinal and obnoxious grasses of Rajasthan, India. J Ethnopharmacol 76:293–297

    Article  CAS  Google Scholar 

  7. Vasanthakumari MM, Shivanna MB (2009) Fungal assemblages in the rhizosphere and rhizoplane of grasses of the subfamily Panicoideae in the Lakkavalli region of Karnataka India. Microb Environ. https://doi.org/10.1264/jsme2.me10163

    Article  Google Scholar 

  8. Nischitha R, Shivanna MB (2020) Influence of seasons on endophytic fungal assemblage in Alloteropsis cimicina (L.) Stapf. and Heteropogon contortus (L.) P. Beauv. of the sub-family panicoideae. Curr Res Environ Appl Mycol J Fungal Biol 10(1):10–25

    Article  Google Scholar 

  9. Bhat KG, Nagendran CR (2001) Sedges and Grasses (Dakshina Kannada and Udupi districts). Dehra Dun, Bishen Singh Mahendra Pal Singh (Ed): 341

  10. Schulz B, Wanke U, Draeger S, Aust HJ (1993) Endophytes from herbaceous plants and shrubs, effectiveness of surface sterilization methods. Mycol Res 9:1447–1450

    Article  Google Scholar 

  11. Arx Von JA (1981) On Monilia sitophila and some families of Ascomycetes. Sydowia Ann Mycologici 34:12–29

    Google Scholar 

  12. Nischitha R, Vasanthkumari MM, Kumaraswamy BE, Shivanna MB (2020) Antimicrobial and antioxidant activities and chemical profiling of Curvularia tsudae endophytic in Cynodon dactylon (L.) Pers. 3 Biotech 10(7):1–12

    Article  Google Scholar 

  13. Nischitha R, Shivanna MB (2021) Antimicrobial activity and metabolite profiling of endophytic fungi in Digitaria bicornis (Lam) Roem. & Schult. and Paspalidium flavidum (Retz.) A. Camus. 3 Biotech 11(2):1–15

    Article  Google Scholar 

  14. Gagana SL, Kumaraswamy BE, Shivanna MB (2020) Diversity, antibacterial and antioxidant activities of the fungal endophytes associated with Schleichera oleosa (Lour.) Merr. S Afr J Bot 134:369–381

    Article  Google Scholar 

  15. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181(4617):1199–1200

    Article  CAS  Google Scholar 

  16. Arulpriya P, Lalitha P, Hemalatha S (2010) Cyclic voltammetric assessment of the antioxidant activity of petroleum ether extract of Samanea saman (Jacq.) Merr. Pelagia. Res Libr 1(3):24–35

    CAS  Google Scholar 

  17. Koleva Y, Georgieve S, Agova N, Iliev I (2019) Molecular properties and bioactivity score of newly synthesized derivatives of bexarotene. Proc Uni Rus 58:2–10

    Google Scholar 

  18. Nayab RS, Maddila S, Krishna MP, Titinchi SJ, Thaslim BS, Chintha V, Chamarthi NR (2020) In silico molecular docking and in vitro antioxidant activity studies of novel α-aminophosphonates bearing 6-amino-1, 3-dimethyl uracil. J Recept Signal Transduct 40(2):166–172

    Article  CAS  Google Scholar 

  19. Vinale F, Nicoletti R, Lacatena F, Marra R, Sacco A, Lombardi N, d’Errico G, Digilio MC, Lorito M, Woo SL (2017) Secondary metabolites from the endophytic fungus Talaromyces pinophilus. Nat Prod Res 31(15):1778–1785

    Article  CAS  Google Scholar 

  20. Koul M, Meena S, Kumar A, Sharma PR, Singamaneni V, Riyaz-Ul-Hassan S, Hamid A, Chaubey A, Prabhakar A, Gupta P, Singh S (2016) Secondary metabolites from endophytic fungus Penicillium pinophilum induce ROS-mediated apoptosis through mitochondrial pathway in pancreatic cancer cells. Planta med 82(04):344–355

    Article  CAS  Google Scholar 

  21. Yasser MM, Marzouk MA, El-Shafey NM, Shaban SA (2020) Diversity and antimicrobial activity of endophytic fungi from the medicinal plant Pelargonium graveolens (geranium) in Middle Egypt. Jordan J Biol Sci 13(2):197

    CAS  Google Scholar 

  22. George TK, Joy A, Divya K, Jisha MS (2019) In vitro and in silico docking studies of antibacterial compounds derived from endophytic Penicillium setosum. Microb Pathogen 131:87–97

    Article  CAS  Google Scholar 

  23. Danagoudar A, Joshi CG, Sunil Kumar R, Poyya J, Nivya T, Hulikere MM, Anu Appaiah KA (2017) Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3. Mycol 8(1):28–38

    Article  CAS  Google Scholar 

  24. Govindappa M, Lavanya M, Aishwarya P, Pai K, Lunked P, Hemashekhar B, Arpitha BM, Ramachandra YL, Raghavendra VB (2020) Synthesis and characterization of endophytic fungi, Cladosporium perangustum mediated silver nanoparticles and their antioxidant, anticancer and nano-toxicological study. Bio Nano Sci 10:1–14

    Google Scholar 

  25. Rebiai A, Lanez T, Belfar ML (2011) In vitro evaluation of antioxidant capacity of algerian propolis by spectrophotometrical and electrochemical assays. Int J Pharmacol 7:113–118

    Article  CAS  Google Scholar 

  26. Amamra S, Cartea ME, Belhaddad OE, Soengas P, Baghiani A, Arrar KI, L, (2018) Determination of total phenolics contents, antioxidant capacity of Thymus vulgaris extracts using electrochemical and spectrophotometric methods. Int J Electrochem Sci 13:7882–7893

    Article  CAS  Google Scholar 

  27. Do E, Lee HG, Park M, Cho YJ, Kim DH, Park SH, Eun D, Park T, An S, Jung WH (2019) Antifungal mechanism of action of lauryl betaine against skin-associated fungus Malassezia restricta. Mycobiol 47(2):242–249

    Article  Google Scholar 

  28. de Oliveira Filho JWG, Andrade TDJADS, de Lima RMT, Silva DHS, Dos Reis AC, Santos JVDO, de Meneses AAPM, de Carvalho RM, da Mata AMO, de Alencar MVOB, Dias ACS (2020) Cytogenotoxic evaluation of the acetonitrile extract, citrinin and dicitrinin-A from Penicillium citrinum. Drug Chem Toxicol. https://doi.org/10.1080/01480545.2020.1769642

    Article  PubMed  Google Scholar 

  29. Ertl P, Rohde B, Selzer P (2000) Fast calculation of molecular polar surface area as a sum of fragment-based contributions and its application to the prediction of drug transport properties. J Med Chem 43:3714–3717

    Article  CAS  Google Scholar 

  30. Singh V, Praveen V, Tripathi D, Haque S, Somvanshi P, Katti SB, Tripathi CKM (2015) Isolation, characterization and antifungal docking studies of wortmannin isolated from Penicillium radicum. Sci Rep 5(1):1–13

    Google Scholar 

Download references

Acknowledgements

The first author thanks the National Fellowship for Scheduled Caste (NFSC), UGC, New Delhi for awarding Senior Research Fellowship and also thankful to SAIF, IIT, Bombay for OHR LC-MS and FTIR service.

Funding

The research was funded by the University Grants Commission (NFSC) RGNF-2017–18-SC-KAR-38457.

Author information

Authors and Affiliations

  1. Department of PG Studies and Research in Botany, School of Biosciences, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga, Karnataka, 577 451, India

    R. Nischitha & M. B. Shivanna

Authors
  1. R. Nischitha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. B. Shivanna
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MBS conceived the initial work idea and NR performed the laboratory experiments and data analysis. MBS and NR worked on the data interpretation of the work. NR prepared the first draft copy of the manuscript and the corrections were made and finalized by MBS and approved the final manuscript.

Corresponding author

Correspondence to M. B. Shivanna.

Ethics declarations

Conflict of interest

The authors declare no competing financial interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 178 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nischitha, R., Shivanna, M.B. Metabolite fingerprinting, in vitro antimicrobial and antioxidant activities and in-silico docking in Alloteropsis cimicina and its endophytic fungus Penicillium pinophilum. Mol Biol Rep 48, 4021–4037 (2021). https://doi.org/10.1007/s11033-021-06410-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-021-06410-0

Keywords

Search

Navigation