Abstract
Sickle cell disease is a group of diseases inherited through the gene and it affects the haemoglobin in the red blood cell. This study investigated the methanol seed extract of Buchholzia coriacea for possible in vitro anti-sickling effects and also determined the effect of Mucuna pruriens seed extract on the solubility and oxygen-binding rate of sickle cell haemoglobin. Sickle cell blood was collected from sickle cell disease patients with subsequent addition of 2% sodium metabisulphite to cause more sickling. Varying concentrations of the seed extracts (50%, 25%, 12.5% and 6.25%) were added to the pre-treated blood for these in vitro assays. The results showed that the extract of Buchholzia coriacea significantly (P < 0.05) inhibited sickling at all concentrations with the highest percentage inhibition of 73.3 ± 5.8, reversed sickled erythrocytes at all concentrations with the highest percentage reversal of 83.3 ± 5.8 and significantly (P < 0.05) inhibited polymerisation at all concentrations used in comparison to the parallel control. The extract of Mucuna pruriens seed significantly (P < 0.05) increased the solubility of sickle haemoglobin at 50%, 25%, 12.5% and 6.25% concentrations, increased Fe2+/Fe3+ ratio from 1.7 (control) to 12.2 (50% concentration) and reduced osmotic fragility (at 12.5% and 6.25% concentrations) when compared with parallel control. The results indicate the feasibility of the seed extracts as promising agents in the management of sickle cell disease.
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Acknowledgements
The authors are grateful to Dr. Christian E. Odoh of Michael Okpara University of Agriculture, Umudike, Nigeria, for his efforts towards the success of this work. They are also grateful to Dr. Ejiofor C. Agbo of Federal Medical Centre, Umuahia, Nigeria, for his help in obtaining the blood samples used in this study.
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Ikechukwu, E.L., Okafor, P.N. & Egba, S.I. In vitro assessment of the anti-sickling properties of Buchholzia coriacea and Mucuna pruriens seed extracts. In Vitro Cell.Dev.Biol.-Animal 56, 773–782 (2020). https://doi.org/10.1007/s11626-020-00512-y
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DOI: https://doi.org/10.1007/s11626-020-00512-y