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Callus and suspension cell cultures of Sutherlandia frutescens and preliminary screening of their phytochemical composition and antimicrobial activity

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Abstract

Callus and suspension cell cultures were successfully developed from Sutherlandia frutescens (Fabaceae), an endemic medicinal plant of South Africa. Two callus cell lines, originating from hypocotyl and cotyledon explants of in vitro seedlings under both dark and light conditions, showed intensive fresh weight accumulation with growth index ranging from 4.6 to 5.9. Suspension cell cultures induced from two callus lines had similar growth profiles and their growth index (15–18), specific growth rate (0.15–0.16 day−1), productivity (0.83–0.96 g/(l day)) and maximum biomass accumulation (16–18 g/l) remained relatively high for Fabaceae cell cultures during 27 sub-cultivations. Callus and suspension cell cultures showed similar profiles of secondary metabolites that were, however, different from leaves of greenhouse plants. Isoflavones were predominant in both callus and suspension cell cultures while flavonoids (sutherlandins) and triterpene glycosides of the cycloartane group (sutherlandiosides) were mostly found in leaves. Nineteen fatty acids (FA), both short- and very-long-chained (up to C25:0), were found in cell cultures. Linoleic and α-linolenic FA together comprised 60–64% out of total FA content in cell cultures followed by palmitic acid (18–25%). Extracts of suspension cell biomass exhibited antimicrobial activity against Staphylococcus aureus but were not effective against Pseudomonas aeruginosa. To the best of our knowledge, this is the first report on the induction, phytochemical composition and antimicrobial activity screening of S. frutescens suspension cell cultures which opens the door for their biotechnological application.

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All data used during the study are available from the corresponding author by request.

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Acknowledgements

The work on investigation of cell culture growth characteristics and secondary metabolite composition was financially supported by the Ministry of Science and Higher Education of Russian Federation through Megagrant project no. 075-15-2019-1882 and performed using the equipment of the large-scale research facilities “All-Russian Collection of cell cultures of higher plants” and “Experimental biotechnological facility” of the Institute of Plant Physiology of Russian Academy of Sciences. The results of cell culture induction and maintenance were obtained within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. 122042700045-3). The results of analysis of FAs composition were obtained within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. 122042700043-9). The results of antimicrobial activity analysis were obtained within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. 122042600086-7).

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  1. Alexander V. Nosov, Maria V. Titova and Dmitry V. Kochkin have contributed equally to this work.

Authors and Affiliations

  1. K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    Alexander V. Nosov, Maria V. Titova, Artem A. Fomenkov, Dmitry V. Kochkin, Roman A. Sidorov, Anastasia A. Medentsova, Elena V. Popova & Alexander M. Nosov

  2. Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia

    Dmitry V. Kochkin & Alexander M. Nosov

  3. Ural Federal University, Yekaterinburg, Russia

    Boris A. Galishev

  4. Gorbatov Federal Research Center of Food Systems, Russian Academy of Sciences, Moscow, Russia

    Elena A. Kotenkova

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  1. Alexander V. Nosov
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Nosov, A.V., Titova, M.V., Fomenkov, A.A. et al. Callus and suspension cell cultures of Sutherlandia frutescens and preliminary screening of their phytochemical composition and antimicrobial activity. Acta Physiol Plant 45, 42 (2023). https://doi.org/10.1007/s11738-023-03526-7

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