Abstract
Detailed anatomical studies of flowers and fruits may render traits of phylogenetic importance, but are still rare in the Boraginales. An Old World clade of Cordia comprises many dioecious species, but the floral anatomy is largely unexplored and, hence, the structural basis for sex separation. Fresh material of buds, functionally unisexual flowers and fruits were studied using a stack shot camera. Fixed such material of Cordia crenata, C. cf. grandicalyx and C. sinensis was sectioned using a microtome and stained with safranin red and astra blue, and the gynoecial surface was studied using SEM. The stamens of the female flowers are largely intact, but do not produce pollen, whereas the gynoecium of the male flowers is highly reduced though develops ovules lacking an embryo sac. The morphologically rather unreduced stamens of the female flower may have undergone a shift of function from producing pollen towards attracting pollinators by optical signals. The outer parenchyma of the gynoecium is strongly stained and after fertilisation, one through three ovules are maturing towards seeds. The presence of more than a single seed enclosed in the undivided endocarp is rare in Cordiaceae.
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Introduction
Dioecy in plants is characterised as reproductive system with distinct female and male individuals. The advantage of dioecy is that self-fertilisation is excluded, and that xeno-gamy (outcrossing) is promoted, to the cost that the sex partners must meet with some risk of their mating occasionally failing (Darwin, 1876). Dioecy is rare in angiosperms, with only accounting for ca 6% of the species (Endress, 1996; Sakai & Weller, 1999; Renner, 2014). Notably, dioecious plants include disproportionately many wind-pollinated species and disproportionately many woody species (Renner & Ricklefs, 1995). The anatomical basis to better understand dioecy on a functional level is still scarce, and only few ontogenetic studies of dioecious plants are available at present.
From an evolutionary perspective, dioecy in angiosperms has originated several times independently (Endress, 1996; Sakai & Weller, 1999; Renner, 2014). It is a dynamic phenomenon, and transformations back to monoecy have also been shown (Himmelreich et al., 2012; Schäfer & Renner, 2010). Dioecy occurs also in the asterids and the group of Boraginales: Lepidocordia Ducke and Rochefortia Sw. from the Ehretiaceae (Irimia & Gottschling, 2016; Miller & Nowicke, 1990) and species (groups) assigned to the two subclades Collococcus P.Browne (Gaviria, 1987; Miller, 1988, 1989) and Myxa (Endl.) Lindl. of Cordia L. from the Cordiaceae (Gottschling et al., 2005; Mez, 1890). Such plants have perfect dimorphic flowers, in which one sex each is functionally suppressed. Knowledge of dioecy in the Boraginales is primarily based on the study of herbarium material, but anatomical (and ecological) approaches are unavailable at present.
Cordiaceae, without exception woody, encounter ca 400 species of worldwide distribution, especially in the tropics and subtropics (Luebert et al., 2016). They belong to the Primarily Woody Boraginales (PWB: Gottschling, 2003) being distinguished by indehiscent fruits and a multi-layered endocarp that bursts at predetermined dehiscence lines during germination (Diane et al., 2002; Gottschling, 2004; Heigl et al., 2020). Cordiaceae are a monophyletic group based on molecular phylogenetics and apomorphies such as the four stigmatic branches of the gynoecium, plicate cotyledons and an undivided endocarp (Gottschling et al., 2005; Heigl et al., 2020; Luebert et al., 2016) and comprise Cordia and Varronia P.Browne (Miller & Gottschling, 2007). As inferred from molecular phylogenetics, Cordia segregates into the subclades Sebestena Boehm., Collococcus and Myxa, whereas the latter two are closer related (Gottschling et al., 2005).
Considerable knowledge has been gained of the developing gynoecium in the PWB (Gottschling, 2004; Gottschling et al., 2014; Heigl et al., 2020; Hilger, 1987, 1992; Jeiter et al., 2018; Pitot, 1939; de Souza, 2008). The internal architecture is complex and results from the development of several septa, including apical and false septa, which promote the formation of the locules and seed chambers. Moreover, basal septa shift the placentae and hence the funiculi to different levels that are ultimately species and group specific. Axial placentation is abundant across Boraginales, and Cordiaceae have hemi(ana)tropous (versus anatropous) ovules as probable apomorphy (Heigl et al., 2020). Of the four initial ovules, one through three are frequently abortive and consequently, many fruits of Cordiaceae contain a single seed only (Luebert et al., 2016).
The overwhelming number of investigations study New World species of Cordiaceae. Here, three dioecious Old World species of Cordia assigned to the Myxa subclade are inspected in detail, namely C. crenata Delile, C. cf. grandicalyx Oberm. and C. sinensis Lam. They comprise small shrubs or trees that are up to 7 m tall (Fig. 1L, M). Cordia cf. grandicalyx (here studied as cultivated plant) is distributed rather restrictedly in Southern Africa (Martins, 1990, 1993), whereas the other two species (here studied based on fixed material collected in the field) have wider geographic ranges in Africa and Asia (Verdcourt, 1991; Warfa, 1989, 1990). Anatomically, the Myxa clade is unexplored, and we aim at better knowledge of the structural basis of dioecy. We compare our results to the available data of other Cordiaceae (Heigl et al., 2020; de Souza, 2008) and close relatives such as Ehretiaceae (Gottschling, 2004; Gottschling et al., 2014), Heliotropiaceae (Hilger, 1987, 1992; Jeiter et al., 2018) and Hydrophyllaceae (Hofmann, 1999; Jeiter & Weigend, 2018; Vasile et al., 2021) to shed light on the rise of an important sexual system present in angiosperms.
Material and methods
The plant material of C. crenata subsp. meridionalis Warfa (Raffeneau-Delile, 1813; Warfa, 1989) was collected from the banks of river Engare Ondare/Kenya (information on the field project is available from https://refubium.fu-berlin.de/handle/fub188/27528) by H.H. Hilger and W. Schultka in January 1986 and comprised male anthetic flowers and older bisexual flowers. Three specimens (Schultka G20!) representing the population are deposited at herbarium FR. The plant material of C. cf. grandicalyx (Obermeijer, 1937) was gathered from a cultivated plant in the Botanical Garden Munich (München-Nymphenburg, accession number 2014–2117) and comprised various stages from female bud to mature fruit. The plant was grown from a seed initially obtained from Sunshine Seeds (Ahlen, Germany; accession number 443370). Several specimens (such as Gottschling & Pätzold HBM00613!) are deposited at herbarium M. Determination of the species is tentatively, as our material somewhat differs from the flower and fruit being more slender and the stylar branches less elongated as described in the protologue (Obermeijer, 1937). However, no other name of a Cordia species would fit the plant better than C. cf. grandicalyx (Martins, 1990, 1993; Verdcourt, 1991). The plant material of C. sinensis (Lamarck, 1792) was collected in Ein Gedi/Israel by H.H. Hilger in May 1980 and comprised female flowers and mature fruits. A specimen (Frey et al. VO3013!) representing the population is deposited at TUB-TAVO.
For macroscopic images, the freshly harvested specimens of C. cf. grandicalyx were photographed with a ILCE-6400 alpha digital camera (Sony; Tokyo, Japan) mounted on a macroscope Z6 APO (Leica; Wetzlar, Germany) with objective lens Planapo 1.0 × (Leica). A series of 40 individual images (under magnifications ranging between 5.7 × and 36 ×) were taken with a stack shot rail (Cognisys; Traverse City, USA–MI) and were rendered with the software Helicon Focus Pro (Helicon Soft; Kharkiv, Ukraine). For light micro-scopy of all species under investigation, the formalin-acetic acid–ethanol (FAA)-fixed buds, flowers and fruits were dehydrated in an ethanol/tertiary butanol series and embedded in Richard-Allan Scientific Paraffin type 9 (Microm International; Walldorf, Germany). Safranin red/astra blue-stained serial sections (7–11 μm) were produced using the RM 2155 microtome (Leica), and images were taken with various (stereo)microscopes (Leica DM1000, Leica DM2000, Leica M80) and digital cameras (Olympus DP73; Tokyo, Japan; Kappa zelos, Kappa DX 20 H-FW; Gleichen, Germany; Casio ex h10; Tokyo, Japan).
For scanning electron microscopy (SEM), the bud primordia (C. cf. grandicalyx) and gynoecia (all species) were dissected under a stereomicroscope, dehydrated in a graded acetone series, ‘critical point-dried’ with liquid CO2 (Cohen & Shaykh, 1973), sputter coated with platinum and analysed with a LEO 438VP scanning electron microscope (LEO; Cambridge, UK). Image adjustments (scaling, cropping, white-balancing, colour management) were done in Adobe®Photoshop (Adobe Systems; Munich, Germany), and images were arranged with Adobe®InDesign (Adobe Systems).
Results
Flower and perianth
Flowers are solitary or composed in small inflorescences comprising up to 15 elements. They are arranged terminal (Fig. 1A–C), ramiflorous (Fig. 1N) or cauliflorous. The basic monotelic inflorescence is an acropetalous, ebracteose thyrsoid comprised of monochasial paracladia with few scattered trichomes. The terminal flower is usually not developed, and the early paracladia are less branched than the younger ones (Fig. 1C). The flowers are shortly pedicellate, subsessile or sessile. In C. cf. grandicalyx, a sweet scent is emitted from the flower, and the cup of the anthetic calyx and corolla is full of nectar.
The flowers of all species under investigation are basically tetracyclic and pentamerous. Only occasionally, they exhibit four or six petals and stamens in C. cf. grandicalyx, or they are trimeric or tetrameric in C. crenata. The calyx is synsepalous and completely fused in bud with no visible lobes (Fig. 1A–C, G). The botuliform or urceolate calyx reaches its final size before anthesis and opens irregularly in C. cf. grandicalyx and C. sinensis (Fig. 1E, F; no data for C. crenata). The surface is glabrous, with a few trichomes accumulating at the apex (Fig. 1A, E–G). In mature fruit, the calyx is dilated but does not enclose the fruit (Fig. 6L).
The petals are fused to a terete tube for half of their lengths (Fig. 1H). The free distal lobes are oblanceolate and involute in C. cf. grandicalyx and C. sinensis (Fig. 1D, N) and triangular in C. crenata (not shown). The colour of the petals at anthesis is creamy white in C. cf. grandicalyx (Fig. 1C, D, K), pale yellow in C. sinensis (Fig. 1N) and creamy in C. crenata (H.H. Hilger, pers. obs.). On the adaxial surface of the corolla and below the free parts of the filaments, white scattered trichomes are found in all species under investigation (Fig. 1K). In C. cf. grandicalyx, dark red spots develop after lesion damage, and the petals turn completely brownish-red after anthesis (Fig. 1H–J). The corolla wilts but remains with the young fruit, until it ultimately falls off at fruit maturity.
Androecium
Stamens are developed, irrespectively of the flowers being functionally male or female (or bisexual as supposed for C. crenata). The filaments are fused to the lower half of the corolla tube in all three species (Fig. 1K). The anthers consist of two thecae and four microsporangia (Fig. 2D, F). They reach their final size already in bud, when the filaments are still short and unstretched (Fig. 2L). The anthers are introrse and dorsifixed and in C. cf. grandicalyx, red and blue spots and lines develop on the abaxial surface (Fig. 1D, K) near the somewhat swollen connective. These markings may correspond to strongly stained tissues in the anatomical sections of C. crenata (Fig. 2A, D, E, H) and C. cf. grandicalyx.
The anther walls consist of three layers, namely an epidermis, an endothecium comprising one cell layer and a secretory tapetum of one to few cell layers. Cells of the endothecium can be recognised by anticlinal, fibrillar wall thickenings (Fig. 2B, E). Intracellular crystals are present along the joint dehiscence lines of two adjacent microsporangia in C. crenata and C. sinensis (visible in phase-contrast microscopy: Fig. 2H, K) but not in C. cf. grandicalyx. The flowers of C. cf. grandicalyx and C. sinensis are functionally female and therefore the microsporangia contain no microspores. The archespore (which usually develops into microspores and tapetum cells nourishing the microspores) remains undifferentiated in both species (Fig. 2F, G, J). In C. crenata, pollen grains originate by meiosis of the microspore mother cells and are already developed early during anthesis. The pollen grains are binucleate when mature (Fig. 2C). The thickened exine represents the outer layer of the pollen grains (Fig. 2B, C).
Gynoecium
A normally developed gynoecium is present in C. cf. grandicalyx (Fig. 3A, B, D, E) and C. sinensis (Fig. 3C, F), whereas it is only rudimentary in the functionally male flowers of C. crenata (Fig. 3J, K). The gynoecium is superior, bicarpellate and coenocarpous-syncarpous in all species, but a style is only developed in functionally female or bisexual flowers. The shape of the gynoecium is obovoid (C. cf. grandicalyx: Fig. 1J) or ovoid (C. sinensis: Fig. 5A). Internally, the synascidiate zone is rather short, and the symplicate region comprises the main part of the gynoecium. The symplicate region corresponds to the ventral slit that is orientated perpendicularly to the coalescence plane of the two carpels (Figs. 3E, F, J; 4N; 6F). It continues distally to the asymplicate zone with the first splitting of the style. The gynoecium is glabrous throughout its entire development.
Stomata in the epidermis of the gynoecium are occasionally found in all three species (Fig. 5). They vary in size, morphology and arrangement with adjacent cells. In C. cf. grandicalyx and C. sinensis, they are found scattered from the base of the gynoecium (Fig. 5E, J) through the base of the style and sometimes even on the proximal style. Stomata are more frequent in the distal than the proximal region of the gynoecium of C. sinensis (Fig. 5A, B). In C. crenata, stomata are found from the base through the equator of the gynoecium. Distal stomata are smaller and appear somewhat vestigial (Fig. 5G) in comparison to the proximal ones (Fig. 5F). The precise function of the stomata remains elusive. An inconspicuous, collar-like disc is present in C. crenata (Fig. 5H) and C. cf. grandicalyx, but is lacking in C. sinensis. No stomata are found on this organ.
In its most distal part, the style separates into four branches and terminates into four papillate stigmata (Figs. 1C, D, H; 3A–C; 5A). Each branch has the length of approximately one-third of the mature gynoecium. The stylar branches are rather narrow in bud and with maturation, the lobes expand and become oblanceolate to obovate in shape of the outline. They are folded in bud and subsequently stretched at anthesis. The transmitting tissue originates right above the level of the funiculi and proceeds through the distal carpels and terminates within the stigmata (Fig. 3C, D).
Each carpel is supplied by a usually prominent dorsal bundle (Figs. 3J, 4N–Q, 6B–D), whereas lateral and ventral bundles are more or less distinctive. The dorsal bundles continue into the stylar vascular bundles (Fig. 4O, P). The primary (or true) septa arise from the fused boundaries of the two involute carpels, each bearing two ovules. The architecture of the functional gynoecium is determined by the presence of different secondary septa, namely four basal septa, two false septa and an apical septum. Only the ventral bundles supply the (true) septa (Fig. 4N). The false septa (Fig. 3E–G, J) continue to the apical septum, and this structure is developed at a 90° angle to the carpels’ coalescence plane. In the male flowers of C. crenata, the apical septum is absent due to the rudimentary development of the carpels’ apices (Fig. 3K), but false septa are nevertheless present (Fig. 3J).
Each primary loculament is twice two-parted by the true and the false septa, resulting in four single ovules each located in its own locule (Figs. 3E, F, J, 4N). In the regions of the funiculi and of the ventral bundles, crystals are found in the cells (Fig. 3G). Each ovule comprises one multi-layered integument with a distinctive micropyle and a nucellus enclosing the embryo sac. In C. cf. grandicalyx, the embryo sac is stalked (Fig. 3H). Ovules in functionally male flowers of C. crenata lack an embryo sac (Fig. 3J, K).
Fruit and seed development
At early ontogenetic states, the corolla tube is ripped (Fig. 1J) and finally falls off the fruit. At maturity, the calyx becomes slightly larger in C. cf. grandicalyx but never encloses the fruit. The remains of the style persist in fruit (Fig. 6L, M). The mature fruit is spherical to ellipsoid in shape. The colour is greenish to yellowish white in C. cf. grandicalyx (Fig. 6M) and orange in C. crenata and C. sinensis.
In all species, the fruit is a drupe (or deriving from it, see description below for C. crenata) preserving the basic architecture of the ovary. Pericarp development is complex, and the course of the dorsal and lateral bundles may help to homologise the different tissues in its various expressions. In postanthetic flowers of C. crenata (Figs. 4D–F, 6C, D, F), the bundles are notably distinctive and embedded in a tissue, whose cells lignify early during fruit maturation corresponding to (an inner portion of) the mesocarp. However, the prospective stony endocarp is still parenchymatic at this ontogenetic stage and only supplied by the ventral bundles (Fig. 6C, D). The outer mesocarp is parenchymatic as well and comprises a minor portion of the maturing fruit (Fig. 6F). The mature endocarp is ellipsoid with a smooth surface (Fig. 6K).
The principal fruit anatomy of C. sinensis is similar to C. crenata. However, the dorsal and lateral bundles are not embedded in lignified tissue but in strongly stained parenchymatic tissue (Figs. 3C, F, 6B). A comparable strongly stained tissue is also found in C. cf. grandicalyx (Fig. 4K–M), but dorsal and lateral bundles are only weakly recognisable in this species. In C. sinensis, the strongly stained tissue is sharply delimited from the remaining pericarp (Figs. 4Q, 6B), but rather scattered within the remaining parenchymatic portions of the pericarp in C. cf. grandicalyx (Figs. 3D, E, 4K–M, 6A, E). The further the fruit develops, this tissue becomes more intensely stained. In C. sinensis, the tissue consists of intact cells (Fig. 4A–C) whereas in C. cf. grandicalyx, cellular borders cannot be inferred leading to a syncytial appearance (Fig. 4G–J). In mature fruits of C. cf. grandicalyx, the portion of the mesocarp directly abutting on the endocarp is flashily pink in stained cross sections (Fig. 6A, E).
All four ovules in functionally female flower have the same size at anthesis, but seeds develop in reduced number, indicated by fruits with one (Fig. 6B, D, F, J), two (Fig. 6A, C, E, H) or three seeds (or at least developed locules: Fig. 6G). Mature seeds consist primarily of the embryo, whereas the endosperm is sparse (Fig. 6G, J). The differential growth of seeds results from the asymmetry of the fruit, particularly of the endocarp. The locules with the abortive ovules are left as small, collapsed slits in the endocarp (Fig. 6G, J).
Dehiscence lines of the endocarp are predetermined already early in development (Fig. 4N, 6A–D, F). Each locule is opened by two such lines, one of which is structurally connected with a dorsal bundle. The mature embryo consists of reniform, plicate cotyledons and the radicula. Two types of cotyledons could be distinguished: In C. cf. grandicalyx, they have a notably long petiole, and the shoot apex is barely above the substrate (Fig. 6N) whereas in C. crenata, the hypocotyl is very long, raising the two shortly petiolate cotyledons well above the substrate (Fig. 6O).
Discussion
Sex distribution and functional transformation
The evolutionary origin of dioecy has attracted generations of researchers including Darwin (1876), Endress (1996) and Renner (2014), but anatomical studies to better understand the phenomenon on a functional level are still scarce. Our observations on three Old World species of Cordia confirm that the reciprocal sex is supressed and/or reduced in dioecious Boraginales, but morphologically not entirely lost (Gaviria, 1987; Irimia & Gottschling, 2016; Mez, 1890; Verdcourt, 1991). As shown in the present study, the functionally male flowers of C. crenata have a rudimentary gynoecium lacking a style, stigmata and distinct embryo sacs.
In the functionally female flowers of C. cf. grandicalyx and C. sinensis (and probably many other Old World species of Cordia: Martins, 1990; Verdcourt, 1991), the shape of the anthers is not rudimentary, but it is the process of meiosis which is suppressed. Moreover, the stamens develop optical signals in form of red and blue spots and lines on the abaxial surface, at least in C. cf. grandicalyx. In other species such as Actinidia polygama (Siebold & Zucc.) Maxim. (Actinidiaceae) and Petasites tricholobus Franch. (Asteraceae), a similar functional transformation has been observed and interpreted as a pollinator attracting syndrome (Kawagoe & Suzuki, 2004; Mayer & Charlesworth, 1991; Willson & Ågren, 1989; Yu et al., 2011) and frequently, the pollinators are rewarded by an extensive amount of nectar. This would be also in agreement with our observations of female flowers in C. cf. grandicalyx, in which much nectar is offered (as it is frequent in other species of Cordia: Askins et al., 1987; Dalsgaard, 2011; McMullen, 2012). Also after our study, the precise structures releasing nectar in C. cf. grandicalyx remain to be determined — gynoecial stomata can be probably excluded, as they are much too small in number.
Both the optical signals of petals and androecium, and the provision of nectar, indicate entomophily, and beetle pollination is reported for the species of Cordia having white or pale flowers (Gaviria, 1987; Kevan et al., 1996). No-tably, entomophilous plants are less frequent across dioecious angiosperms than anemophilous ones (Renner & Ricklefs, 1995). Furthermore, we cannot exclude at this moment in time that the dimorphic flowers of Cordia are result of an ontogenetic sex change (Endress, 1996; plasticity sensu Charnov & Bull, 1977). To the best of our knowledge, individuals of Cordia have yet not been observed over a longer period of time, and more research is necessary to enlighten the morphological and anatomical basis of sex separation in Cordia, putatively relying on seasonal or temporal factors (Condon & Gilbert, 1988; Schlessman, 1988; Sakai & Weller, 1999).
Gynocecium
A strongly stained tissue in the periphery of the gynoecium is a trait of C. cf. grandicalyx and C. sinensis [but not of C. crenata; it is also absent from Cordia trichotoma (Vell.) Arrab. ex Steud. from the Sebestena subclade: de Souza, 2008]. Similar tissue was found before only in C. nodosa from the Collococcus subclade (Heigl et al., 2020), but identity and function remains elusive. It is worthy to note that in other Boraginales, the dorsal and lateral bundles take their course in the prospective mesocarp, but not in the endocarp (Gottschling, 2004; Gottschling et al., 2014; Heigl et al., 2020; Hilger, 1987, 1992; Pitot, 1939). In a comparative approach, the course of those bundles may thus help to elucidate the homology of the strongly stained tissues between the species based on the criterion of position.
In C. nodosa, the strongly stained tissue is sharply delimited from the adaxial regions of the gynoecium, and bundles take their course clearly outside the tissue, whereas they are rather embedded in the strongly stained tissue of C. sinensis. The tissue of C. cf. grandicalyx is different from the other two species, as it is not sharply delimited and is not cellular but appears syncytial. The flashily pink tissue (based on an intensive safranin staining by identical treatment of younger fruits and flowers) surrounding the endocarp in the prospective fruit may indicate the presence of lipids, which has not been reported from any species of Cordia so far. Cordia crenata is again unusual with the lignification of parenchyma internally of the bundle ring, and gynoecial and fruit architecture appears as an evolutionarily very dynamic trait in Cordia.
Two assumptions about the deviation of the strongly stained tissue have been made, either originating from the epidermis or resulting from a growing disc that surrounds the gynoecium and the style (Heigl et al., 2020). The presence of nectarostomata stated for Hydrophyllaceae (Hofmann, 1999; Jeiter & Weigend, 2018; Vasile et al., 2021) was indicative for this ‘disc’ interpretation but in Cordia, only regular stomata are found scattered over the gynoecial surface. Moreover, the assumption may appear also less likely in the light of the Old World species’ anatomy studied here, as the tissue is by far not that sharply delimited as in New World C. nodosa (if such various tissues are homologous at all). The alternate explanation of a multilayered coat is considered a xeromorphy (Shields, 1950), and many species of Cordia grow in at least seasonally very dry habitats. More research is necessary to gain broader knowledge of the strongly stained tissue and its occurrence across species of Cordia, the homology, the evolution and the function.
Other morphological deviations
The basic tetracyclic architecture is the same in all species of Cordia studied here, but the number of sepals, petals and stamens is varying between three and six, with pentamerous flowers as the predominant type. This confirms the great plasticity present in flowers of Cordia (Miller, 1988, 1990, 2013; McMullen, 2012; Martínez-Adriano et al., 2016; Heigl et al., 2020). Embryologically, a stalked embryo sac can be found in C. cf. grandicalyx, which is the second report in Cordiaceae after the first of Varronia bonplandii Desv. (Heigl et al., 2020). Function or advantage of a pedunculate embryo sac is still unclear. Since Varronia is the sister group of Cordia, and other species such as C. nodosa (Heigl et al., 2020) and C. trichotoma (de Souza, 2008) do not have a stalked embryo sac, this character may have developed independently and could be an autapomorphy of C. cf. grandicalyx.
Drupes are the most abundant fruit type in Cordiaceae (Gottschling et al., 2016; Verdcourt, 1991) and sometimes, nut-like fruits are developed (Miller, 2013). However, C. crenata is highly unusual when portions of the mesocarp start to lignify already in anthetic flowers. At this point in time, the prospective endocarp is still parenchymatic and not yet lignified. Irrespectively of this anatomical distinctiveness, the fruit develops to a red-coloured drupe at maturity (Warfa, 1989; Verdcourt, 1991) as in other species of Cordia. Moreover, the number of seeds per fruit is frequently one in Cordiaceae (Heigl et al., 2020; Luebert et al., 2016; de Souza, 2008). All three species studied here show deviations from this rule: Usually, more than a single ovule develops, and fruits regularly contain two if not three seeds (Martins, 1990; Mez, 1890). A complete set of four seeds per fruit is abundant in Ehretiaceae and Heliotropiaceae (Gottschling, 2004; Gottschling et al., 2014; Hilger, 1992; Pitot, 1939), and the larger number of seeds per fruit in the Myxa subclade of Cordia might thus be interpreted as plesiomorphic reminiscence.
Seedling morphology of Cordiaceae is poorly known at present and does not go beyond anecdotal reports (Gaviria, 1987; Lubbock, 1892; Mensbruge, 1966; Warfa, 1988). To the best of our knowledge, the very short hypocotyl together with the very long petioles is a unique combination of characters in C. cf. grandicalyx. Future research should put attention to other putative close relatives in order to enlighten whether more species of Cordia share this seedling morphology with C. cf. grandicalyx. However, other members of the Myxa subclade such as C. sinensis have the abundant type with long hypocotyl and short cotyledon petioles.
Conclusion
The morphological and anatomical studies render many characters, which confirm the correct systematic placement of C. crenata, C. cf. grandicalyx and C. sinensis in Cordia. The four stigmatic branches, the undivided endocarp and the plicate cotyledons are clear apomorphies of Cordiaceae (Gottschling et al., 2005; Luebert et al., 2016) that are also present in the three species studied here. Course and extent of the various gynoecial septa, placentation being axial and the hemi(ana)tropous ovules further found in the present study are also in accordance with previous results of Cordiaceae (Heigl et al., 2020; de Souza, 2008). Other characters such as the strongly stained, peripheral parenchyma of the gynoecium, or the number of seeds developed per fruit, must be studied for additional species of Cordia to draw more precise phylogenetic conclusions. Regarding the structural basis for the evolution of dioecy, the possible shift of androecial function from producing pollen towards attracting pollinators in Cordia is the most unexpected result presented here. Our study underlines the need of morphological and anatomical investigations for an improved evolutionary and functional knowledge of Cordiaceae.
Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Code availability
Not applicable.
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Acknowledgements
We thank Harald Loose with his team (Munich), who devotedly cares for the living plants at the Botanical Garden. Stefan Dressler (Frankfurt) and Uta Grünert (Tübingen) provided digital images of herbarium specimens, which is gratefully acknowledged here. We also thank Andreas Fleischmann (Munich) for discussion of characters and ecologies relating to the research presented here and Eva Facher (Munich) for assistance in the lab.
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Marc Gottschling and Janin Pätzold contributed to the study conception and design. Material preparation, data collection and analysis were performed by Janin Pätzold, Benedikt Feyrer, Johanna Saumweber, Marc Gottschling and Hartmut H. Hilger. Janin Pätzold arranged the plates. All authors interpreted and discussed the anatomical and morphological findings. The first draft of the manuscript was written by Marc Gottschling and Janin Pätzold, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Pätzold, J., Feyrer, B., Saumweber, J. et al. Observations on flower and fruit anatomy in dioecious species of Cordia (Cordiaceae, Boraginales) with evolutionary interpretations. Org Divers Evol 23, 77–90 (2023). https://doi.org/10.1007/s13127-022-00576-6
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DOI: https://doi.org/10.1007/s13127-022-00576-6