Skip to main content
SpringerLink
Log in

Phytochemical analysis and determination of antioxidant, anti-cholesterol, anti-inflammatory and anti-proliferative activities of Gerbera jamesonii flowers

  • Research Article
  • Published:
Advances in Traditional Medicine Aims and scope Submit manuscript

Abstract

From the flowers of Gerbera jamesonii, eleven compounds have been isolated for the first time and identified as; apigenin (1), kampferol (2), quercetin (3), dihydroquercetin (Taxifolin) (4), quercetin-4`-O-β-d-glucopyranoside (5), rutin (6), 4-β-d-glycopyranosyloxy-5-methylcoumarin (Gerberinside) (7), 5,6-dihydro-4-β-d-glycopyranosyloxy-6-methyl-2 H-pyran-2-one(Gerberin) (8), 3,4-dihydroxybenzoic acid (Protocatechuic acid) (9), stigmasterol (10) and β-sitosteryl-3-O-β-d-glucopyranoside (Daucosterol) (11). The structures have been established by comparison of their NMR and MS data with published ones. Free radical scavenging activity of flower extracts was further investigated, which indicated potent antioxidant activity ranged from 94.4 ± 1.16 to 99.3 ± 0.15 using 16.7 mg/ml of each extract; the IC50 of all extracts was abstemiously low between 3 and 7 mg/ml. All extracts were able to degrade cholesterol with different potencies ranged from 64.96% ± 1.87 to 69.89% ± 4.02 after 96 h of incubation. Compound (6) has a potent anti-inflammatory activity even with low concentration (90.3 ± 0.57, 94.55 ± 0.78, 97.4 ± 1.13, using 0.001, 0.0015 and 0.005 mg, respectively). Concerning anti-proliferative activity, all fractions exerted 100% anti-tumor activity using 1 mg of each sample against HCT-116, MCF-7 human cancer cells and good anti-tumor activity against HepG-2 human cancer cells exceeding Doxorubicin in the case of petroleum ether extract with no cytotoxic activity against BJ-1 human skin healthy cell-line. This could promote the use of G. jamesonii flower extract for pharmacological and health purposes. For author’s knowledge, this is the first report evaluating the biological activities of G. jamesonii flowers extract.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Achika J, Arthur D, Gerald I, Adedayo A (2014) A review on the phytoconstituents and related medicinal properties of plants in the Asteraceae family. IOSR J Appl Chem 7:1–8

    Article  CAS  Google Scholar 

  • Bjelakovic G, Gluud LL, Nikolova D, Bjelakovic M, Nagorni A, Gluud C (2011) Antioxidant supplements for liver diseases. The Cochrane database of systematic reviews. Issue 3. Art. No.: CD007749

  • Chou CT (1997) The anti-inflammatory effect of Tripterygium wilfordi hook on adjuvant induced paw edema in rats and inflammatory mediator’s release. Phytother Res 17:152–154

    Article  Google Scholar 

  • Cohen SJ (2001) Overdose. ISBN: 1-58542-123–5

  • Crawford DJ (1978) Flavonoid chemistry and angiosperm evolution. Bot Rev 44:431–456

    Article  CAS  Google Scholar 

  • Ejebe DE, Siminialayi IM, Emu dainowho JO et al (2013) Analygesic and anti- inflammatory activities of the ethanol extract of the leaves of Helianthus Annus in Wister rats. Asian Pac J Trop Med 3:341–347

    Google Scholar 

  • Ellison S (2005) Health myths exposed. Copyright 2005. ISBN: 1-4208-0027–2.M.Sc. Courtesy

  • Fossen T, Pedersen AT, Andersen QM (1998) Flavonoids from red onion (Allium cepa). Phytochemistry 47(2):281–285

    Article  CAS  Google Scholar 

  • Hamburger M, Hostettman K (1991) Bioactivity in plants: The link between phytochemistry and medicine. Phytochemistry 30:3864–3874

    Article  CAS  Google Scholar 

  • Hansen HV (1985) A taxonomic revision of the genus Gerbera (Compositae, Mutisieae) sections Gerbera, Parva, Piloselloides (in Africa), and Lasiopus. (Opera botanica. No. 78). ISBN 87-88702-04-9

  • Harborne JB (1994) The flavonoids, advances in research since 1986, vol 116–238. Chapman and Hall, London, New York, pp 446–471

    Book  Google Scholar 

  • He F, Wang M, Gao M, Zhao M, Bai Y, Zhao C (2014) Chemical composition and biological activities of Gerbera anandria. Molecules 19:4046–4057

    Article  PubMed  PubMed Central  Google Scholar 

  • Heywood H, Harborne JB, Turner BL 1977 The Biology and chemistry of the compositae. By V Academic Press, Vol. I, London. www.floraqueen.com/blog/gerbera-daisy-care-the-vibrant-beauty

  • Lee JH, Ku CH, Baek N, Kim SH, Park HW, Kim DK (2004) Phytochemical constituents from Diodia teres. Arch Pharm Res 27(1):40–43

    Article  CAS  PubMed  Google Scholar 

  • Lee B, Kim J, Kang YM et al (2010) Antioxidant activity and γ–aminobutyric acid (GABA) content in sea tangle fermented by Lactobacillus brevis BJ20 isolated from traditional fermented foods. Food Chem 122:271–276

    Article  CAS  Google Scholar 

  • Lin YL, Wang WY, Kuo YH, Chin CF (2000) Nonsteroidal constituents from Solanum incanum L. J Chin Chem Soc 47:247–251

    Article  CAS  Google Scholar 

  • Lykkesfeldt J, Svendsen O (2007) Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J 173:502–511

    Article  CAS  PubMed  Google Scholar 

  • Manson JE, Tosteson H, Ridker PM, Sattereld S, Herbert P, O’Conner GT (1992) The primary prevention of myocardial infarction. N Engl J Med 326:1406–1416

    Article  CAS  PubMed  Google Scholar 

  • Miremadi F, Ayyash M, Sherkat F, Stojanovska L (2014) Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic lactobacilli and bifidobacteria. J Funct Foods 9:295–305

    Article  CAS  Google Scholar 

  • Mohammed HA, Abdelwahab MF, El-Ghaly EM, Ragab EA (2021) Phytochemical characterization, in vitro anti-inflammatory, anti-diabetic, and cytotoxic activities of the edible aromatic plant; Pulicaria jaubertii. Molecules 26:203

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nagumo S, Toyonaga T, Inoue T, Nagai M (1989) New glucoside of a 4- hydroxy-5-methylcoumarin and a dihydro-α-pyrone from Gerbera jamesonii hybrid. Chem Pharm Bull 37(10):2621–2623

    Article  CAS  Google Scholar 

  • Negm El-Dein A, Nour El-Deen AM, El-Shatoury EH et al (2021) Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic‎ potential of some Egyptian Lactobacillus spp. Int J Biol Macromol 173:66–78

    Article  Google Scholar 

  • Nikolić M, Stevović S (2015) Family Asteraceae as a sustainable planning tool in phytoremediation and its relevance in urban areas. Urban For Urban Green 14(4):782–789

    Article  Google Scholar 

  • Pan D, Zhang D (2005) Screening of cholesterol–reducing lactic acid bacteria and its activity in cholesterol–reducing. Food Sci 26(6):233–237

    CAS  Google Scholar 

  • Prasad KN, Cole WC, Kumar B, Prasad KC (2002) Pros and cons of antioxidant use during radiation therapy. Cancer Treat Rev 28:79–91

    Article  CAS  PubMed  Google Scholar 

  • Ragab EA, Raafat M (2016) A new monoterpene glucoside and complete assignments of dihydroflavonols of Pulicaria jaubertii: potential cytotoxic and blood pressure lowering activity. Nat Prod Res 30(11):1280–1288

    Article  CAS  PubMed  Google Scholar 

  • Sadique JA, Al-Rqobah WA, Bugharith ME, El-Gin-dy AR (1989) The bioactivity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia 6:525–532

    Google Scholar 

  • Scott KN (1972) Carbon-13 nuclear magnetic resonance of biologically important aromatic acids. I. Chemical shifts of benzoic acid and derivatives. J Am Chem Soc 29: 1972

  • Simone CB, Simone NL, Simone V, Simone CB (2007) Antioxidants and other nutrients do not interfere with chemotherapy or radiation therapy and can increase kill and increase survival, part 1. Alternat Ther Health Med 13:22–28

    Google Scholar 

  • Voutquenne L, Lavaud C, Massiot G, Sevenet T, Hadi HA (1999) Cytotoxic polyisoprenes and glycosides of long-chain fatty alcohols from Dimocarpus fumatus. Phytochemistry 50:63–69

    Article  CAS  PubMed  Google Scholar 

  • Ye JN, Wang TZ, Cai SQ et al (1990) Pharmacological studies on folk medicine in Sichuan province, China II: on Tu-er-fang derived from gerbera plants. J Pharmac Soc Jpn 110(6):374–382

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research work was accomplished in Pharmacognosy Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt and Chemistry of Natural and Microbial Products Department and Tanning Materials and Leather Technology Department, National Research Centre, Egypt.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Pharmacognosy Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

    Shaimaa Negm El-Dein, Amal Hussein, Marwa S. Abu-Bakr & Ehab A. Ragab

  2. Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt

    Asmaa Negm El-Dein

  3. Tanning Materials and Leather Technology Department, National Research Center, Dokki, Giza, Egypt

    Hanem M. Awad

Authors
  1. Shaimaa Negm El-Dein
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amal Hussein
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marwa S. Abu-Bakr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Asmaa Negm El-Dein
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hanem M. Awad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ehab A. Ragab
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shaimaa Negm El-Dein.

Ethics declarations

Ethical statement

This article does not contain any studies involving animals or human participants performed by any of the authors.

Conflict of interest

Shaimaa Negm El‑Dein has no conflict of interest. Amal Hussein has no conflict of interest. Marwa S. Abu‑Bakr has no conflict of interest. Asmaa Negm El‑Dein has no conflict of interest. Hanem M. Awad has no conflict of interest. Ehab A. Ragab has no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Material 1

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Negm El-Dein, S., Hussein, A., Abu-Bakr, M.S. et al. Phytochemical analysis and determination of antioxidant, anti-cholesterol, anti-inflammatory and anti-proliferative activities of Gerbera jamesonii flowers. ADV TRADIT MED (ADTM) 23, 863–875 (2023). https://doi.org/10.1007/s13596-022-00659-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13596-022-00659-x

Keywords

Search

Navigation