Abstract
Nauclea pobeguinii (N. pobeguinii) is a plant used in African medicine to treat many gastroduodenal diseases. In this study, we determined the gastro-protective mechanisms and anti-Helicobacter pylori (H. pylori) properties of N. pobeguinii extracts. Wound healing activity (acetic acid test), anti-secretory properties (pyloric ligation, pyloric ligation/acetylcholine and pyloric ligation/histamine tests) and cytoprotective effects (ethanol test) were assessed in female rat, the anti-Helicobacter pylori (agar well diffusion method) was also evaluated. At doses of 100, 200 and 400 mg/kg, the extracts reduce (p < 0.001) the various ulceration parameters. In the acetic acid test, the extracts (200 mg/kg) reduced ulcerated areas by 99.23% (aqueous) and by 98.47% (methanol), levels of monocytes, lymphocytes, nitrogen, malondialdehyde and increased (p < 0.001) superoxide dismutase and catalase activities. Histological analysis showed repair of the mucosal epithelium at all doses of both extracts. Aqueous and methanol extracts inhibited ulceration indices by 99.68 and 99.33% (pyloric ligation), 83.81% and 61.07% (pyloric ligation/acetylcholine), 97.49% and 98.50% (pylorus ligation/histamine); they increased (p < 0.001) the mucus mass and uterine mass. In vitro, the different H. pylori isolates were sensitive to both extracts; the aqueous extract showed strong anti-urease activity, a large diameter of the inhibitory zone and a better minimum inhibitory concentration. Aqueous and methanolic extracts of N. pobeguinii healed ulcers through their estrogen-modulating anti-inflammatory, antioxidant, anti-secretory and cytoprotective properties. The aqueous extract of N. pobeguinii could be a good solution for the treatment of this infection.
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MKYK, AG and MM designed the work. MKYK, AG, MM, FZNL, NAE, TEG, DNSF, ACF and MMVM conducted the work and collected and analysed the data. MKYK, AG and MM drafted the manuscript and revised it critically. All authors agree to be accountable for all aspects of the work.
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The experimental procedures have been approved by the local ethics committee and are in accordance with the guidelines for the study of pain in awake animals, published by the NIH (publication no. 85-23), “Principles of Animal Protection,” Laboratory, Study of Pain, Ministry of Scientific Research and Technology, which adopted the European Union Guidelines on Animal Care and Experimentation (EWC 86/609).
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MKYK is PhD students in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. MM (PhD) is a senior lecturer in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. AG is an associate professor in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. FZNL, NAE, TEG, DNSF, ACF and MMVM are PhD students in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. The authors declare that they have no conflicts of interest.
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Yacine Karelle, M.K., Marius, M., Zenab Linda, F.N. et al. Gastro-protective effects and anti-Helicobacter pylori activities of the aqueous and methanol extracts of the stem-back of Nauclea pobeguinii (Rubiaceae). ADV TRADIT MED (ADTM) 24, 223–242 (2024). https://doi.org/10.1007/s13596-023-00686-2
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DOI: https://doi.org/10.1007/s13596-023-00686-2