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Pollen morphology of Malpighiaceae from Brazilian forest fragments

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Abstract

The pollen morphology of 12 Brazilian native species of Malpighiaceae from forest fragments was investigated in search to expand the morphological knowledge of the species analyzed thus contributing to its palynology, taxonomy, and conservation. The pollen grains were acetolysed, measured, photographed using light microscopy and scanning electron microscopy, and described qualitatively. The quantitative data were analyzed by descriptive statistics and multivariate statistics. The pollen grains are monads, apolar or isopolar, small to large, with circular to quadrangular amb, oblate-spheroidal to prolate-spheroidal, tricolporate with long colpi and lalongate endoaperture or 6–(7–8)-porate with or without colpoids and aspides, psilate-rugulate, rugulate or microreticulate exine, sexine thicker than nexine in most species. The results of this study point to differences in the morphology of the pollen grains in the analyzed species, which indicates Malpighiaceae as a eurypalynous family; this fact was also confirmed by quantitative data and the multivariate analysis. These data reinforce the importance of pollen morphology for identification and distinction of genus and species of the family.

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References

  • Amorim AM (2002) Five new species of Heteropterys (Malpighiaceae) from Central and South America. Brittonia 54:217–232

    Article  Google Scholar 

  • Amorim AM (2003) Estudos taxonômicos em Heteropterys (Malpighiaceae). PhD Thesis, São Paulo University

  • Anderson WR (1981) Malpighiaceae. In: Maguire B (ed) The Botany of the Guayana Highland-Part XI. Mem New York Bot Gard 32:21–305

  • Anderson C (1982) A monograph of the genus Peixotoa (Malpighiaceae). Contrib Univ Mich Herb 15:1–92

    Google Scholar 

  • Anderson WR (1990) The origin of the Malpighiaceae: the evidence from morphology. Mem N Y Bot Gard 64:210–224

    Google Scholar 

  • Anderson WR (1993) Chromosome number of neotropical Malpighiaceae. Contrib Univ Mich Herb 19:341–354

    Google Scholar 

  • Anderson WR (2004) Malpighiaceae (Malpighia family). In: Smith N, Mori SA, Henderson A, Stevenson DW, Heald SV (eds) Flowering plants of the neotropics. Princeton University Press, New Jersey, pp 229–232

    Google Scholar 

  • Apg III (2009) An update of the phylogeny group classification for the orders and families of flowering plants: APG III. Bot J Linn Soc 161:105–121

    Article  Google Scholar 

  • Barth OM, Melhem TS (1988) Glossário ilustrado de Palinologia. Editora da Universidade Estadual de Campinas, Campinas

    Google Scholar 

  • Cameron KM, Chase MW, Anderson WR, Hillis HG (2001) Molecular systematics of Malpighiaceae: evidence form plastid rbcL and matK sequences. Am J Bot 88:1832–1847

    Article  Google Scholar 

  • Davis CC, Anderson WR (2010) A complete generic phylogeny of Malpighiaceae inferred from nucleotide sequence data and morphology. Am J Bot 97:2031–2048

    Article  PubMed  Google Scholar 

  • Davis CC, Anderson WR, Donoghue MJ (2001) Phylogeny of Malpighiaceae: evidence from chloroplast ndhF and trnL-F nucleotide sequences. Am J Bot 88:1830–1846

    Article  CAS  PubMed  Google Scholar 

  • Davis CC, Bell CD, Mathews S, Donoghue MJ (2002a) Laurasian migration explains Gondwanan disjunctions: evidence from Malpighiaceae. Proc Natl Acad Sci 99:6833–6837

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Davis CC, Bell CD, Fritsch PW, Mathews S (2002b) Phylogeny of Acridocarpus-Brachylophon (Malpighiaceae): implications for tertiary tropical floras and Afroasian biogeography. Evolution 56:2395–2405

    Article  CAS  PubMed  Google Scholar 

  • Davis CC, Fritsch PW, Bell CD, Mathews S (2004) High latitude tertiary migrations of an exclusively tropical clade: evidence from Malpighiaceae. Int J Plant Sci 165:S107–S121

    Article  CAS  Google Scholar 

  • Dutra FV, Santos HD, Ribeiro PC, Gasparino EC (2014) Morfologia polínica em espécies ornamentais de Asteraceae, Ericaceae, Fabaceae, Malpighiaceae, Malvaceae e Rubiaceae. Nucleus 11:7–17

    Article  Google Scholar 

  • Erdtman G (1952) Pollen morphology and plant taxonomy–angiosperms. Alquimist & Wiksell, Stockholm

    Google Scholar 

  • Erdtman G (1960) The acetolysis method. A revised description. Sven Bot Tidskr 54:561–564

    Google Scholar 

  • Faegri G, Iversen J (1966) Textbook of modern pollen analysis, 2nd edn. Scandinavian University Books, Copenhagen

    Google Scholar 

  • Gasparino EC, Cruz-Barros MAV, Chautems A (2013) Pollen morphology in Brazilian species of Codonanthe (Mart.) Hanst. and Nematanthus Schrader (Gesneriaceae). Grana 52:274–285

    Article  Google Scholar 

  • Gonçalves-Esteves V, Soares Júnior EF, Mendonça CBF (2007) Palinologia de espécies de Malpighiaceae Juss. ocorrentes nas restingas do Estado do Rio de Janeiro. Hoehnea 34:519–529

    Article  Google Scholar 

  • Judd WS, Campbell CS, Kellogg EA, Stevens PF, Donoghue MJ (2009) Sistemática Vegetal: um enfoque filogenético, 3a edn. Artmed, Porto Alegre

    Google Scholar 

  • Kronka FJN, Matsukuma CK, Nalon MA, Delcali IH, Rossi M, Mattos IFA, Shinike MS, Pontinhas AAS (1993) Inventário Florestal do Estado de São Paulo. Instituto Florestal, São Paulo

    Google Scholar 

  • Lobreau D (1967) Contribution à l’etude du pollen des Malpighiaceae d’Afrique. Pollen Spores 9:241–277

    Google Scholar 

  • Lobreau D (1968) Le pollen des Malpighiacées d’Afrique et de Madagascar. Étude sur la systematique des genres à la lumiére de nouvelles observations palynologigues. Bull I FAN 30:59–83

    Google Scholar 

  • Lobreau-Callen D (1983) Analyse de la répartition géographique des Malpighiaceae d’après les caracteres du pollen et de la pollinisation. Bothalia 14:871–881

    Article  Google Scholar 

  • Magalhães-e-Silva FH (2007) Contribuição à palinologia das Caatingas. PhD Thesis, State University of Feira de Santana, Feira de Santana

  • Makino WH (1986) Flora polínica da Reserva do Parque Estadual das Fontes do Ipiranga – Família 125-Malpighiaceae. Hoehnea 13:21–30

    Google Scholar 

  • Makino-Watanabe H (1988) Contribuição ao estudo palinológico das Malpighiaceae A.L. Jussieu do Brasil (Tribo Banisterieae, Subtribo Banisteriinae). PhD Thesis, State University of Campinas

  • Makino-Watanabe H, Melhem TS, Barth MO (1993a) Morfologia dos grãos de pólen de espécies de Banisteriopsis C.B. Robinson ex Small (Malpighiaceae). Rev Bras Bot 16:47–67

    Google Scholar 

  • Makino-Watanabe H, Melhem TS, Barth MO (1993b) Morfologia dos grãos de pólen de espécies brasileiras de Janusia A. Juss. e Schwannia Endl. (Malpighiaceae). Hoehnea 20:79–86

    Google Scholar 

  • Makino-Watanabe H, Melhem TS, Barth MO (1998) Morfologia polínica de Camarea St.-Hil. (Malpighiaceae). Rev Bras Bot 21:1–6

    Article  Google Scholar 

  • Mamede MCH, Amorim AMA, Sebastiani R, Almeida RF (2014) Malpighiaceae. In: Forzza RC (coord) Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB155. Accessed Aug 2014

  • McCune B, Mefford MJ (1999) PC-ORD. Multivariate analysis of ecological data. MjM Software Design, Oregon

    Google Scholar 

  • Melhem TS, Cruz-Barros MAV, Corrêa AMS, Makino-Watanabe H, Silvestre-Capelato MSF, Gonçalves-Esteves VL (2003) Variabilidade polínica em plantas de Campos do Jordão (São Paulo, Brasil). Bol Inst Bot 16:1–104

    Google Scholar 

  • Myers N, Mittermeier RA, Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858

    Article  CAS  PubMed  Google Scholar 

  • Niendenzu F (1928) Malpighiaceae. In: Engler A (ed) Das Pflanzenreich. Verlag von Wilhelm Engelmann, Leipzig, pp 247–572

    Google Scholar 

  • Punt W, Hoen PP, Blackmore S, Nilsson S, Le Thomas A (2007) Glossary of pollen and spore terminology. Rev Paleobot Palynol 143:1–81

    Article  Google Scholar 

  • Ranga NT, Rezende AA, Cavasan O, Toniato MTZ, Cielo-Filho R, Stranghetti V (2012) Caracterização florística de remanescentes de vegetação nativa da região noroeste do Estado de São Paulo. In: Necchi O Jr (ed) Fauna e Flora de fragmentos florestais remanescentes da região Noroeste do Estado de São Paulo. Holos Editora, Ribeirão Preto, pp 105–135

    Google Scholar 

  • Rao SRS, Sarma V (1992) Morphology of 2-armed trichomes in relation to taxonomy: malpighiales. Feddes Repert 103:559–565

    Article  Google Scholar 

  • Robertson KR (1972) The malpíghiaceae in the southeastern United States. J Arnold Arbor 53:101–112

    Google Scholar 

  • Roubik DW, Moreno JE (1991) Pollen and spores of Barro Colorado Island. Missouri Botanical Garden, New York

    Google Scholar 

  • Salgado-Labouriau ML (1973) Contribuição à Palinologia dos Cerrados. Academia Brasileira de Ciências, Rio de Janeiro

    Google Scholar 

  • Salgado-Labouriau ML, Vanzolini PE, Melhem TS (1965) Variation of polar axés and equatorial diameters in pollen grains of two species of Cassia. Grana Palynol 6:98–105

    Google Scholar 

  • Sebastiani R, Cruz-Barros MAV, Corrêa MAS (2014) Palynological study of Janusia A. Juss. and related genera (Malpighiaceae). Braz J Bot. doi:10.1007/s40415-014-0082-1

    Google Scholar 

  • Shepherd GJ (1996) Fitopac 1: manual do usuário. Departamento de Botânica. Universidade Estadual de Campinas, Campinas

    Google Scholar 

  • Sousa SM, Reis AC, Gomes SSL, Bernardo KB, Salimena FRG, Viccini LF (2010) Botanical aspects of Heteropterys umbellata (Malpighiaceae): a cytological and palynological approach. An Acad Bras Ciênc 82:869–879

    Google Scholar 

  • Souza CN, Gasparino EC (2014) Pollen morphology of Fridericia Mart. (Bignoniaceae) from Brazilian forest fragments. Braz J Bot 37:83–94

    Article  Google Scholar 

  • Turner IM (1996) Species loss in fragments of tropical rain forest: a review of the evidence. J Appl Ecol 33:200–209

    Article  Google Scholar 

  • Vieira S (1981) Introdução à Bioestatística. Campus, Rio de Janeiro

    Google Scholar 

  • Zar JH (1996) Biostatistical analysis, 2th edn. Prentice Hall, New Jersey

    Google Scholar 

Download references

Acknowledgments

The authors thank the State of São Paulo Research Foundation (FAPESP—2013/11832-7) for the scholarship of the first author, Prof. Dr. F.J. Zara (DBAA–FCAV) by granting the optical photomicroscope used, and Profa. Dra. Luciane O. Crossetti (Instituto de Biociências—UFRGS) by help with the PCA programs.

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Authors and Affiliations

  1. Departamento de Biologia aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV), Univ. Estadual Paulista, Unesp, Jaboticabal, SP, 14.884-900, Brazil

    Talita Kely Belonsi & Eduardo Custódio Gasparino

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  1. Talita Kely Belonsi
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  2. Eduardo Custódio Gasparino
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Correspondence to Eduardo Custódio Gasparino.

Appendix: Specimens examined

Appendix: Specimens examined

Banisteriopsis argyrophylla (A. Juss.) B. Gattes: BRASIL. São Paulo: Novo Horizonte, 25 Mar 2010, N.T. Ranga et al. F03 (SJRP); BRASIL. São Paulo: Sales, 24 May 2008, N.T. Ranga et al. 19 (SJRP); BRASIL. São Paulo: Votuporanga, 15 Mar 2008, N.T. Ranga et al. 26 (SJRP).

Banisteriopsis muricata Cavanilles: BRASIL. São Paulo: Barretos, 05 Mar 2009, N.T. Ranga et al. 21 (SJRP); BRASIL. São Paulo: Taquaritinga, 12 Mar 2009, N.T. Ranga et al. 02 (SJRP).

Banisteriopsis nummifera (A. Juss.) B.Gates: BRASIL. São Paulo: Jaboticabal, 14 Aug 1991, E.H.A. Rodrigues 141 (JABU).

Banisteriopsis oxyclada (A. Juss.) B. Gattes: BRASIL. São Paulo: Ida Iolanda, 30 Jun 2006, N.T. Ranga et al. 45 (SJRP); BRASIL. São Paulo: Macaubal, 05 Apr 2008, N.T. Ranga et al. 01 (SJRP); BRASIL. São Paulo: Onda Verde, 01 Jan 2007, N.T. Ranga et al. 14 (SJRP).

Byrsonima crassifolia (L.) Kunth: BRASIL. Minas Gerais: São Roque de Minas, Serra da Canastra, 14 Jun 2009, B.F. Bueno 005 (JABU).

Byrsonima intermedia A. Juss.: BRASIL. Goiás: Rio Verde, 11 May 2009, A.C. Lofego s/n. (SJRP 30024); BRASIL. Mato Grosso: Itiquira, 03 Jun 2003, R.D. Daud & P.R. Demite 37 (SJRP); BRASIL. São Paulo: Barretos, 21 Aug 2008, N.T. Ranga et al. 24 (SJRP); BRASIL. São Paulo: Bebedouro, 19 Feb 2009, N.T. Ranga et al. 28 (SJRP).

Byrsonima verbascifolia (L.) Rich.: BRASIL. Goiás: Edealina, 11 May 2009, A.C. Lofego s/n. (SJRP 30017); BRASIL. Mato Grosso: Itiquira, 03 Jun 2003, R.D. Daud & P.R. Demite 50 (SJRP).

Diplopterys pubipetala (A. Juss.) W. R. Anderson & C. C. Davis: BRASIL. São Paulo: Birigui, Fazenda Água Branca, 11 Aug 1999, Montilha et al. 31 (SJRP); BRASIL. São Paulo: Sales, Fazenda Água Clara, 24 Aug 1995, M.D.N. Grecco et al. 105 (SJRP).

Heteropterys aceroides Griseb.: BRASIL. São Paulo: Paulo de Faria, Estação Ecológica de Paulo de Faria, 23 Jun 1993, V. Stranghette 234 (SJRP); BRASIL. São Paulo: Paulo de Faria, Estação Ecológica de Paulo de Faria, 12 Jan 1994, V. Stranghetti 251 (SJRP).

Mascagnia cordifolia (A. Juss.) Griseb.: BRASIL. São Paulo: Campinas, Distrito de Souzas, 22 Jun 1996, K. Santos s/n. (SJRP 30104); BRASIL. São Paulo: José Bonifácio, antiga Fazenda Jacaré, 07 Apr 1995, M.R. Pietrobom da Silva 2270 (SJRP); BRASIL. São Paulo: São José do Rio Preto, Mata dos Macacos, 1 May 1999, F. Tomasetto s/n. (SJRP 29374).

Peixotoa tomentosa A. Juss.: BRASIL. São Paulo: Itirapina, cerrado de Itirapina, 07 May 1962, Homero do Amaral s/n (JABU514).

Stigmaphyllon lalandianum A. Juss.: BRASIL. São Paulo: Jaboticabal, 09 Mar 1991, E.H.A. Rodrigues 129 (JABU).

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Belonsi, T.K., Gasparino, E.C. Pollen morphology of Malpighiaceae from Brazilian forest fragments. Braz. J. Bot 38, 379–393 (2015). https://doi.org/10.1007/s40415-015-0134-1

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