Abstract
This section describes our endeavor to provide biochemical validation and to demonstrate the efficacy of plants used in the treatment of tuberculosis (TB) in the past as recorded in the PLM, and those used for treatment of TB symptoms by contemporary healers, through in vitro laboratory analyses. The research process consists of several steps, starting with the collection of the plant materials, extraction using a standardized chemical solvent, setting up mycobacterial bacterial culture, submission of plant extracts to biological assay against virulent Mycobacterium tuberculosis H37Rv, recollection of the active samples for bioactivity-guided fractionation, and eventually, for the elucidation of chemical structures of the active molecules against Mtb.
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Elkington, B.G., Soejarto, D.D., Sydara, K. (2014). Biochemical Validation. In: Ethnobotany of Tuberculosis in Laos. SpringerBriefs in Plant Science. Springer, Cham. https://doi.org/10.1007/978-3-319-10656-4_3
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DOI: https://doi.org/10.1007/978-3-319-10656-4_3
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