IAC 201CS and IAC 201CO: Crotalaria cultivars with high fresh matter yield and seed production CULTIVAR RELEASE IAC 201CS and IAC 201CO: Crotalaria cultivars with high fresh matter yield and seed production Crop Breeding and Applied Biotechnology 21(2): e36292129, 2021 Brazilian Society of Plant Breeding. Printed in Brazil http://dx.doi.org/10.1590/198470332021v21n2c38 Sara Regina Silvestrin Rovaris1, Gabriel de Moraes Cunha Gonçalves1*, Jean Fausto de Carvalho Paulino2, Simone Daneluz Gobbi3, Alisson Fernando Chiorato1 and Sérgio Augusto Morais Carbonell1 Abstract: The cultivars IAC 201CS and IAC 201CO have a mean cycle of 100 and 110 days, mean 1000 seed weight of 18 and 6 grams, mean total fresh matter weight of 26,287.81 and 36,174.12 kg ha-1, and mean seed weight of 812.57 and 1,017.09 kg ha-1, respectively. Keywords: Crotalaria spectabilis Roth, Crotalaria ochroleuca G., plant breeding, green manure. INTRODUCTION Crotalaria constitutes one of the largest genera of the Fabaceae family, with around 690 species distributed across tropical and subtropical regions, mainly in the southern hemisphere (Garcia et al. 2013). According to Pereira (2006), Crotalaria (rattlebox/rattlepod) rapidly grows to protect bare soil and provides large biomass production in a short period of time. As a green manure, it supplies a large amount of nitrogen to the soil, helps to control weed growth, and reduces the nematode population (Timossi et al. 2016). Crotalaria ochroleuca G. is an annual leguminous shrub with a determinate, upright growth habit. It adapts well to soil and climate conditions and, like Crotalaria juncea L., has an upright semi-woody stem. It has narrow leaves, an expressive proportion of stem in shoot biomass composition, and small seeds of reniform shape of various colors (Silva et al. 2016). It is susceptible to attack of the rattlebox moth Utetheisa ornatrix L. that, depending on intensity, can compromise seed production (Barreto and Fernandes 2001). Crotalaria spectabilis Roth is a shrub plant of upright determinate growth habit, with a relatively early cycle, though initial development is slow, and it may reach a height of 1.0 to 1.5 m (Aguiar et al. 2014). It is used as a cover crop or green manure crop, like most Crotalarias, but also effectively acts as a trap plant in control of root-knot nematodes in infested soils (Leal et al. 2012, Aguiar et al. 2014). Under high temperature and moisture conditions, a fungal disease, septoria leaf spot/blotch (Septoria crotalariae), can arise in C. spectabilis, causing spots on leaves and early leaf drop, even leading to death of the plant (Maringoni et al. 2012). According to Araújo et al. (2015), mulch from C. spectabilis crop residue interferes in the population dynamics of coco-grass (Cyperus rotundus), with reduction in the population and in the dry biomass of that weed. *Corresponding author: E-mail: gabriel_demoraes@hotmail.com ORCID: 0000-0001-9745-0929 Received: 27 December 2021 Accepted: 08 March 2021 Published: 20 July 2021 Instituto Agronômico de Campinas, Centro de Análises e Pesquisa Tecnológica do Agronegócio dos Grãos e Fibras, 13020-902, Campinas, SP, Brazil 2 Instituto Agronômico de Campinas, Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, 13.020-902, Campinas, SP, Brazil 3 Empresa Mato-grossense de Pesquisa, Assistência e Extensão Rural, 78.550-258, Sinop, MT, Brazil Crop Breeding and Applied Biotechnology - 21(2): e36292129, 2021 1 1 SRS Rovaris et al. As there is no supply of Crotalaria cultivars of the genera C. spectabilis and C. ochroleuca, the Instituto Agronômico (IAC) aimed to develop Crotalaria cultivars to meet market demand for new alternatives of leguminous plants for use as green manure. The cultivars IAC 201CO (C. ochroleuca) and IAC 201CS (C. spectabilis) show high potential for fresh matter and seed production. GENETIC ORIGIN AND DEVELOPMENT The Crotalaria ochroleuca cultivar IAC 201CO was developed by IAC. In November 2017, natural populations of Crotalaria ochroleuca belonging to the active germplasm bank (Banco Ativo de Germoplasma - BAG) of IAC were sown in Campinas, São Paulo. From these populations, 400 plants were selected, based on characteristics of fresh matter production (vigor) and the occurrence of rattlebox moth caterpillars Utetheisa ornatrix L. After evaluation of grain yield, 31 progenies from the plants selected in the previous year were sown in Campinas in November 2018. These 31 progenies were identified as Gen CO 1 to Gen CO 31. From them, eight superior lines of C. ochroleuca were selected. In April 2019, the 8 lines were sown in Campinas and Mococa, São Paulo, in a randomized block experimental design, with four replications, for preliminary evaluation and seed multiplication. In October to December 2019, eight lines plus a check variety, corresponding to the traditional genotype sown by farmers, were sown in trials. The trials were conducted in six evaluation locations, three in the state of São Paulo: Campinas, Votuporanga, and Mococa, and three in the state of Mato Grosso: Canarana, Sinop, and Sorriso (on two dates) under a randomized block experimental design, with four replications. The area used for data collection was 4 m2 per plot. Due to its yield performance, the experimental line denominated Gen CO 23 was selected and given the commercial name IAC 201CO. The Crotalaria spectabilis cultivar, IAC 201CS, was developed by IAC. In November 2017, natural populations of Crotalaria spectabilis belonging to the BAG of IAC were sown in Campinas, São Paulo. From these populations, 300 plants were selected based on the characteristics of fresh matter production (vigor) and the occurrence of disease (Septoria crotalariae). After evaluation of grain yield, 37 progenies from plants selected in the previous year were sown in Campinas in November 2018. These 37 progenies were identified as Gen CS 1 to Gen CS 37. From these progenies, 18 superior lines were selected. In April 2019, the 18 lines were sown in Campinas and Mococa, São Paulo, in a randomized block experimental design, with four replications, selecting nine superior lines. In October, November, and December 2019, the nine lines of C. spectabilis plus a check cultivar, corresponding to the traditional genotype sown by farmers, were placed in trials for determination of plant technological qualities in six locations, three in the state of São Paulo: Campinas, Votuporanga, and Mococa, and three in the state of Mato Grosso: Canarana, Sinop, and Sorriso, in a randomized block experimental design, with four replications. The area used for data collection was 4 m2 per plot. Due to its yield performance, the experimental line denominated Gen CS 37 was selected and given the commercial name IAC 201CS. YIELD POTENTIAL The mean production values of total fresh matter weight (TFMW) considering all the environments in which the trials were conducted exhibited variation from 26,287.81 to 22,645.11 kg ha-1 for the C. spectabilis lines (Table 1). For C. ochroleuca (Table 2), the mean values of TFMW oscillated from 36,174.12 to 24,812.41 kg ha-1. The line Gen CS 37 had a high mean value of TFMW (26,287.81 kg ha-1), which was significant, and it exceeded the production of the check variety by 16% (Table 1). The same situation was observed for the line Gen CO 23, which exhibited a mean value of 36,174.12 kg ha-1 for TFMW, statistically exceeding the check variety. Regarding mean seed weight (SW), considering the mean values obtained in all the environments, the C. spectabilis lines ranged from 812.57 to 606.50 kg ha-1. Once more, the Gen CS 37 line was highest, with a mean SW of 812.57 kg ha-1, 29% higher than the check variety (Table 3). In relation to C. ochroleuca, the Gen CO 23 line exceeded seed production of the control variety by 13%, with a SW of 1,017.09 kg ha-1 (Table 4). Due to their higher mean values for TFMW and for SW, the Instituto Agronômico (IAC) selected the Gen CS 37 line of C. spectabilis and named it IAC 201CS, and the Gen CO 23 line of C. ochroleuca and named it IAC 201CO. The name of the 2 Crop Breeding and Applied Biotechnology - 21(2): e36292129, 2021 IAC 201CS and IAC 201CO: Crotalaria cultivars with high fresh matter yield and seed production cultivars regarding the IAC abbreviation corresponds to the Instituto Agronômico de Campinas; 20 is related to the year of registration in the Brazilian Ministry of Agriculture’s National Cultivar Registry (MAPA/RNC); 1 is the number of registry of both Crotalaria species by IAC; and CS and CO correspond to the C. spectabilis and C. ochroleuca species, respectively. The cultivars IAC 201CS and IAC 201CO were registered in the National Cultivar Registry (Registro Nacional de Cultivares - RNC) of the Brazilian Ministry of Agriculture (Ministério da Agricultura, Pecuária e Abastecimento - MAPA) on November 17, 2020, under number 46304 and 46303, respectively. OTHER CHARACTERISTICS The IAC 201CS cultivar has an annual cycle, with flowering at an average of 70 days and maturation at an average of 100 days after emergence. Mean plant height was from 1.0 to 1.5 meters, and mean 1000 seed weight was 18 g. A Table 1. Mean total fresh matter weight (TFMW) in kg ha-1, coefficient of variation (CV%), and least significant difference (LSD, Dunnett – p<0.05) of genotypes of Crotalaria spectabilis Roth considering six evaluation locations, as well as the combination of environments, 2019/20 crop year Genotype of Crotalaria spectabilis Gen CS 37 Gen CS 3 Gen CS 27 Gen CS 4 Gen CS 28 Gen CS 21 Gen CS 26 Gen CS 17 Gen CS 32 Check Variety Overall Mean CV (%) LSD (kg ha-1) Campinas 45,093.75 42,987.50 44,456.25 40,343.75 41,375.00 38,693.75 38,418.75 35,337.50 41,025.00 34,356.25 40,208.75 12.03 9,856.00 Mococa 19,061.88 15,317.50 16,110.63 15,091.88 16,058.75 14,531.25 15,273.75 14,423.75 12,002.50 15,935.00 15,380.68 18.34 5,744.22 TFMW (kg ha-1) Analysis by location Votuporanga Sorriso 38,381.25 32,656.25 38,281.25 29,812.50 32,618.75 30,250.00 34,250.00 29,593.75 36,050.00 27,843.75 34,875.00 30,562.50 33,687.50 29,312.50 35,268.75 29,056.25 31,662.50 28,812.50 34,737.50 29,093.75 34,981.25 29,699.37 14.61 9.71 10,409.50 5,872.96 Sinop 12,727.50 15,088.75 12,265.63 15,538.75 13,095.00 12,030.63 12,711.88 13,359.38 8,755.00 9,607.50 12,518.00 17.83 4,546.88 Canarana 9,806.25 11,384.38 12,378.13 12,565.63 11,993.75 14,409.38 9,715.63 11,603.13 13,228.13 12,140.63 11,922.50 17.81 4,325.11 Combined Analysis 26,287.81* 25,478.64* 24,679.89 24,563.95 24,402.7 24,183.75 23,186.66 23,174.79 22,580.93 22,645.11 24,118.43 14.69 2,770.75 * Dunnett test (p<0.05). Table 2. Mean total fresh matter weight (TFMW) in kg ha-1, coefficient of variation (CV%), and least significant difference (LSD, Dunnett – p<0.05) of genotypes of Crotalaria ochroleuca G. considering six evaluation locations, as well as the combination of environments, 2019/20 crop year Genotype of Crotalaria ochroleuca Gen CO 23 Gen CO 29 Gen CO 2 Gen CO 5 Gen CO 19 Gen CO 18 Gen CO 15 Gen CO 12 Check variety Overall mean CV (%) LSD (kg ha-1) Campinas Votuporanga 111.643.50 93.127.37 63.786.37 69.915.62 101.523.25 99.320.62 79.623.75 84.010.75 79.289.50 86.915.64 5.83 10.263.60 37.625.62 37.015.00 26.288.12 27.546.25 47.495.62 23.581.87 31.054.37 27.865.00 40.262.37 33.577.85 13.31 8.160.20 TFMW (kg ha-1) Analysis by location Sorriso (Nov/2019) Sorriso (Dec/2019) 23.639.37 22.836.25 25.967.50 24.804.37 21.690.62 21.094.37 22.509.37 24.742.50 24.651.25 23.777.50 19.247.50 23.213.75 22.139.37 22.620.00 24.172.50 24.753.75 23.001.25 22.176.87 23.002.08 23.335.48 12.94 9.31 6.018.15 4.397.66 Sinop Canarana Combined Analysis 7.405.00 6.499.37 4.351.25 5.222.50 6.895.00 8.141.87 4.994.37 5.968.75 8.313.75 6.421.32 15.21 1.976.16 13.895.00 12.781.87 11.663.75 12.900.00 10.593.12 13.943.75 14.900.00 13.903.75 13.603.25 13.131.61 5.99 1.593.06 36.174.12* 33.365.91 24.812.41 27.139.37 35.822.62* 31.241.56 29.221.98 30.112.42 31.107.83 30.999.80 9.96 2.386.91 * Dunnett test (p<0.05). Crop Breeding and Applied Biotechnology - 21(2): e36292129, 2021 3 SRS Rovaris et al. Table 3. Mean seed weight (SW) in kg ha-1, coefficient of variation (CV%), and least significant difference (LSD, Dunnett – p<0.05) of genotypes of Crotalaria spectabilis Roth considering six evaluation locations, as well as the combination of environments, 2019/20 crop year Genotype of Crotalaria spectabilis Campinas 306.73 404.82 314.48 360.71 356.03 307.12 313.69 337.18 312.93 289.47 330.31 9.10 61.24 Gen CS 4 Gen CS 37 Gen CS 28 Gen CS 17 Gen CS 32 Gen CS 27 Gen CS 3 Gen CS 21 Gen CS 26 Check variety Overall mean CV (%) LSD (kg ha-1) Mococa 1.776.12 966.50 1.220.63 1.158.13 1.333.13 828.13 1.825.00 976.88 1.408.13 1.367.37 1.286.00 15.14 396.71 SW (kg ha-1) Analysis by location Votuporanga Sorriso 1.208.66 663.49 1.513.61 629.21 1.473.39 664.08 1.325.82 453.13 1.430.38 511.16 1.976.22 494.94 946.86 475.09 1.412.72 366.44 1.152.68 346.99 1.199.26 346.72 1.363.96 495.12 11.17 18.02 310.23 181.77 Sinop 61.89 55.89 91.27 86.26 73.35 64.72 41.79 60.49 35.57 72.67 64.39 8.08 10.60 Canarana 721.50 1,305.37 760.31 850.00 421.65 541.27 652.37 485.31 395.62 485.47 661.88 21.28 286.95 Combined Analysis 789.73* 812.57* 754.02* 705.67 687.61 702.05 709.13 606.50 608.65 626.82 700.87 18.30 100.33 * Dunnett test (p<0.05). Table 4. Mean seed weight (SW) in kg ha-1, coefficient of variation (CV%), and least significant difference (LSD, Dunnett – p<0.05) of genotypes of Crotalaria ochroleuca G. considering seven evaluation locations, as well as the combination of environments, 2019/20 crop year Genotype of Crotalaria ochroleuca Gen CO 23 Gen CO 29 Gen CO 2 Gen CO 5 Gen CO 19 Gen CO 18 Gen CO 15 Gen CO 12 Check variety Overall mean CV (%) LSD (kg ha-1) Campinas Mococa 140.47 199.52 221.48 176.13 188.10 175.00 206.83 187.37 237.54 192.49 6.32 24.62 464.19 494.25 346.41 237.18 313.98 376.74 428.36 363.36 220.46 360.55 15.39 112.23 SW (kg ha-1) Analysis by location Sorriso Votuporanga (Nov/2019) Sorriso (Dec/2019) 2.245.83 2.134.04 1.665.73 2.504.37 1.875.53 1.550.58 2.432.50 2.303.18 1.662.38 2.223.25 1.686.51 1.134.68 2.331.71 2.211.09 1.463.42 2.154.37 2.651.39 1.387.69 2.060.00 1.267.65 1.675.54 1.677.50 1.832.91 1.599.97 1.225.83 2.606.78 1.634.10 2.095.04 2.063.23 1.530.45 7.59 8.39 12.28 321.66 350.42 380.29 Sinop Canarana Combined Analysis 292.20 219.43 269.39 330.81 153.58 266.73 180.39 297.24 157.70 240.83 15.22 74.13 177.17 158.91 168.28 162.82 137.92 157.91 157.97 156.57 172.37 161.10 6.70 21.86 1.017.09* 1.000.37* 1.057.66* 850.19 971.40 1.024.26* 853.82 873.56 893.54 949.10 12.29 83.17 * Dunnett test (p<0.05). between-row spacing of 50 cm and 15-18 plants per linear meter is recommended, resulting in a mean population of 300.000 to 360.000 plants per hectare. The IAC 201CO cultivar has an annual cycle with flowering at an average of 82 days and maturation at an average of 110 days after emergence. Mean plant height was from 1.5 to 2.0 meters and mean 1000 seed weight was 6 grams. A between-row spacing of 50 cm and 15-18 plants per linear meter is recommended, resulting in a mean population of 300.000 to 360.000 plants per hectare. TECHNICAL RECOMMENDATIONS AND SEED PRODUCTION Sowing of the IAC 201CS e IAC 201CO cultivars is recommended in October/November for the South/Southeast region in the states of SC, RS, PR, SP, and MG, and for the Central West region in the states of MT, MS, GO, TO, and DF. 4 Crop Breeding and Applied Biotechnology - 21(2): e36292129, 2021 IAC 201CS and IAC 201CO: Crotalaria cultivars with high fresh matter yield and seed production REFERENCES Aguiar TEA, Gonçalves C, Paterniani MEAGZ, Tucci MLS and Castro CEF (2014) Instruções agrícolas para as principais culturas econômicas. Instituto Agronômico, Campinas, 452p. 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