Medicinal Uses, Phytochemistry and Pharmacological Properties of Elaeodendron transvaalense
Abstract
:1. Introduction
2. Medicinal Uses of Elaeodendron transvaalense
3. Phytochemical Constituents of Elaeodendron transvaalense
4. Pharmacological Activities
4.1. Antibacterial Activities
4.2. Anti-Fungal Activities
4.3. Anti-HIV Activities
4.4. Anti-Inflammatory Activities
4.5. Antioxidant Activities
4.6. Antiplasmodial Activities
4.7. Anti-Protozoan Activities
4.8. Anti-pyretic Activities
4.9. Hypoglycaemic Activities
4.10. Larvicidal Activities
4.11. Cytotoxicity and Mutagenic Activities
5. Conclusion
Author Contributions
Funding
Conflicts of Interest
References
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Medicinal Use | Parts of the Plant Used | Country | References |
---|---|---|---|
Abdominal pains | Bark and roots | Zimbabwe | [24] |
Anthelmintic | Root bark | South Africa | [22,38] |
Arthritis | Root bark | Botswana | [39] |
Backache | Root bark | Botswana | [25,39] |
Bladder infections | Bark | South Africa | [22] |
Blood cleanser | Roots | South Africa | [29] |
Body pains | Bark | South Africa | [20] |
Candidiasis | Roots | South Africa | [40] |
Chest pains | Roots mixed with bulb of Drimia elata Jacq. | South Africa | [41] |
Cough | Bark | South Africa | [22] |
Diabetes | Bark | South Africa | [21] |
Diarrhoea | Bark | South Africa and Swaziland | [10,18,19,20,21,22,23] |
Emetic | Stem | Swaziland | [19,27] |
Female infertility | Bark or roots mixed with Peltophorum africanum Sond. bark and decoction taken orally | South Africa | [33] |
Fever | Bark | South Africa | [10,20,21,23,26,29] |
Haemorrhoids | Root bark | South Africa | [22,38] |
High blood pressure | Root bark mixed with roots of Ozoroa paniculosa (Sond.) R. Fern. & A. Fern. | Botswana | [42] |
High blood pressure | Roots | Botswana | [43] |
HIV/AIDS | Roots | South Africa | [16,44,45] |
HIV/AIDS | Roots mixed with Drimia elata Jacq. bulb, roots of Elephantorrhiza elephantina (Burch.) Skeels and Zanthoxylum capense (Thunb.) Harv., bark of Sclerocarya birrea (A. Rich.) Hochst. and Sarcostemma viminale (L.) R. Br. twigs | South Africa | [34] |
Induce vomiting | Stem bark | South Africa | [46,47] |
Intestinal cramps | Bark | South Africa | [10,18,20,21] |
Kidney infections | Bark | South Africa | [22] |
Laxative | Bark | South Africa | [22] |
Malaria | Bark | South Africa | [48] |
Menorrhagia | Root bark | Botswana, South Africa and Zimbabwe | [20,24,25,49] |
Menstrual problems | Root bark mixed with roots of Ozoroa paniculosa | Botswana | [42] |
Sexually transmitted infections (STIs) | Roots and stem bark | South Africa | [16,45,46,50] |
Sexually transmitted infections (STIs) | Roots mixed with Drimia elata bulb, roots of Elephantorrhiza elephantina and Zanthoxylum capense, bark of Sclerocarya birrea and Sarcostemma viminale twigs | South Africa | [35] |
Skin infections, inflammations and rashes | Bark | Namibia, South Africa and Swaziland | [19,20,30,31,32] |
Sore throat | Leaves | South Africa | [32] |
Stomach aches | Bark and roots | Swaziland and South Africa | [19,20,21,22,23,26,27,28,29] |
Stomach cleanser | Bark | South Africa | [10] |
Venereal diseases | Root bark | South Africa | [22,38] |
Wounds | Bark | Namibia | [51] |
Ethnoveterinary Medicine | |||
Diarrhoea | Bark and leaves | South Africa | [36,37] |
Worms | Leaves | South Africa | [37] |
Mineral and Phytochemical Composition | Values | Plant Parts | References |
---|---|---|---|
Al (mg/kg dry weight (dw) | 26.5–41.6 | Stem bark | [55] |
As (mg/kg dw) | 0.06 | Stem bark | [55] |
Cr (mg/kg dw) | 4.8 | Stem bark | [55] |
Cu (mg/kg dw) | 2.8–3.5 | Stem bark | [55] |
Fe (mg/kg dw) | 59.0–206.0 | Stem bark | [55] |
Flavonoid (mg/catechin equivalents/g dw) | 0.1–0.2 | Stem bark | [48,55] |
Hg (mg/kg dw) | 2.4–8.2 | Stem bark | [55] |
Mn (mg/kg dw) | 11.3–12.7 | Stem bark | [55] |
Ni (mg/kg dw) | 1.8–2.6 | Stem bark | [55] |
Pb (mg/kg dw) | 1.2 | Stem bark | [55] |
Proanthocyanidin (mg/g) | 0.25 | Bark | [48] |
Sn (mg/kg dw) | 40.2–42.1 | Stem bark | [55] |
Sulphur hydryl (µg/g) | 0.36 | Bark | [48] |
Tannin (mg/mL gallic acid equivalent) | 0.4–0.8 | Leaves | [56] |
Total phenolics (mg of gallic acid equivalent/g of extract) | 0.04–9.4 | Bark and leaves | [48,55,57] |
Zn (mg/kg dw) | 3.8–4.4 | Stem bark | [55] |
Phytochemical Compound | Extract | Plant Part | References |
---|---|---|---|
Carbohydrate | |||
Galactitol | Ethanol | Root bark | [52] |
Flavonoid | |||
4’-O-methyl-epigallocatechin | Dichloromethane, ethanol and water | Stem bark | [39,53,54] |
Peltogynoid | |||
(+)-11,11-dimethyl-1,3,8,10-trahydroxy-9-methoxypeltogynan | Ethanol | Root bark | [52] |
Triterpenes | |||
3-oxo-28-hydroxylbetuli-20(29)-ene | Hexane: ethyl acetate | Bark | [23] |
3,28-dihydroxylbetuli-20(29)-ene | Hexane: ethyl acetate | Bark | [23] |
30-hydroxylup-20(29)-ene-3-one | Dichloromethane and water | Root bark | [54] |
6β-hydroxylup-20(29)-ene-3-one | Dichloromethane and water | Root bark | [54] |
6β-hydroxy-lup-20(30)-en-3-one | Ethanol and hexane | Root bark | [52] |
Canophyllal | Ethanol and hexane | Root bark | [52] |
Canophyllol | Ethanol and hexane | Root bark | [52] |
Lup-20(30)-ene-3α,29-diol | Dichloromethane, ethanol and water | Stem bark | [46,53,54] |
Lup-20(29)-ene-30-hydroxy-3-one | Ethanol | Stem bark | [46,53] |
β-sitosterol | Ethanol | Stem bark | [53] |
Ψ-taraxastanonol | Ethanol | Stem bark | [53] |
Activity Tested | Extract | Plant Part | Model | Effect | Reference |
---|---|---|---|---|---|
Antibacterial | Methanol | Bark | Kirby-bauer disk diffusion | Exhibited activities with zone of inhibition of 23 mm and 25 mm to 31 mm against Staphylococcus aureus and Staphylococcus epidermis, respectively | [23] |
Antibacterial | Methanol | Bark | Micro-dilution technique | Minimum inhibitory concentration (MIC) values varied between 0.6 μg/mL and 0.02 μg/mL and extracts inhibited 6% of Staphylococcus aureus and 2% Staphylococcus epidermidis at a minimum concentration of 0.02 μg/mL | [23] |
Antibacterial | Aqeous | Bark | Plate-hole diffusion and broth microdilution methods | Extracts exhibited activities with MIC values of 17.2 mg/mL against both Staphylococcus epidermidis and Staphylococcus aureus | [31] |
Antibacterial | Methanol | Bark | Plate-hole diffusion and broth microdilution methods | Extracts exhibited activities with MIC values of 1.3 mg/mL and 2.5 mg/mL against Staphylococcus epidermidis and Staphylococcus aureus, respectively | [31] |
Antibacterial | Ethanol | Bark | Serial broth microdilution | Extracts exhibited activities with MIC values of 12.5 mg/mL, 1.6 mg/mL and 3.1 mg/mL against Gardnerella vaginalis, Neisseria gonorrhoeae and Oligella ureolytica | [46] |
Antibacterial | Dichloromethan | Bark | Micro-titre plate broth two-fold serial dilution assay | Extracts exhibited activities with MIC values of 0.4 mg/mL against Pseudomonas aeruginosa, 0.5 mg/mL against Staphylococcus aureus and Staphylococcus epidermidis, Escherichia coli, (0.7 mg/mL), Shigella sonnei (0.8 mg/mL) and Salmonella typhimurium (1.0 mg/mL) | [54] |
Antibacterial | Methanol | Bark | Micro-titre plate broth two-fold serial dilution assay | Extracts exhibited activities with MIC value of 1.3 mg/mL against Escherichia coli, Staphylococcus aureus and Salmonella typhimurium, 1.0 mg/mL against Pseudomonas aeruginosa and Shigella sonnei, and Staphylococcus epidermidis (1.7 mg/mL) | [54] |
Antibacterial | Ethanol | Stem bark | Microdilution assay | Extracts exhibited activities with MIC values of 3.1 mg/mL and 0.78 to 1.6 mg/mL against Escherichia coli and Staphylococcus aureus, respectively | [55] |
Antibacterial | Aqueous | Bark | Microdilution assay | Extracts exhibited activities with MIC values of 0.8 mg/mL and 0.2 mg/mL against Bacillus subtilis and Staphylococcus aureus, respectively | [58] |
Antibacterial | Ethanol | Bark | Microdilution assay | Extracts exhibited activities with MIC values of 0.2 mg/mL and 0.1 mg/mL against Bacillus subtilis and Staphylococcus aureus, respectively | [58] |
Antibacterial | Aqueous | Bark | Disc-diffusion assays | Extracts exhibited activities with MIC values of 0.2 mg/mL and 0.3 mg/mL against Bacillus subtilis and Staphylococcus aureus, respectively | [58] |
Antibacterial | Ethanol | Bark | Disc-diffusion assays | Extracts exhibited activities with MIC values of 0.2 mg/mL and 0.6 mg/mL against Bacillus subtilis and Staphylococcus aureus, respectively | [58] |
Antibacterial | Methanol | Roots | Disc diffusion method | Exhibited activities with zone of inhibition of 23 mm against Bacillus cereus, 8 mm against Bacillus pumilus, Staphylococcus aureus, Enterococcus cloacae, Escherichia coli, Aeromonas hydrophila, Proteus mirabilis and Salmonella cholera-suis and 10 mm against Bacillus subtilis, Enterococcus faecalis, Pantoea agglomerans and Pseudomonas aeruginosa | [59] |
Antibacterial | Methanol | Roots | Microdilution method | Exhibited activities with MIC values of 12 mg/mL against Bacillus pumilus, Bacillus subtilis, Enterococcus cloacae and Escherichia coli, 6 mg/mL against Klebsiella pneumoniae, Staphylococcus aureus and Salmonella cholera-suis >12 mg/mL against Aeromonas hydrophila, Proteus mirabilis and Serratia marcescens | [59] |
Antibacterial | Aqueous | Bark | Agar dilution method | Extracts exhibited activities with MIC values of 50.0 mg/mL against Bacillus cereus and Bacillus pumilus, 20.0 mg/mL against Bacillus subtilis and Staphylococcus aureus | [60] |
Antifungal | Methanol | Bark | Plate-hole diffusion and broth microdilution methods | Extract exhibited activities with MIC value of 20.2 mg/mL | [61] |
Antifungal | Hexane | Bark | Agar diffusion assay | Exhibited activities with zone of inhibition of 12 mm to 16 mm against Candida albicans, Candida krusei (8 mm to 14 mm) and Cryptococcus neoformans (14 mm to 16 mm) | [22] |
Antifungal | Hexane | Bark | Microdilution assay | Exhibited activities with MIC values of 0.5 mg/mL against Candida albicans and 1.9 mg/mL against both Candida krusei and Cryptococcus neoformans | [22] |
Antifungal | Hexane | Bark | Microdilution assay | Exhibited activities with minimum fungicidal concentration (MFC) values of 3.8 mg/mL against Candida albicans, Candida krusei (7.5 mg/mL) and Cryptococcus neoformans (1.9 mg/mL) | [22] |
Antifungal | Hexane | Bark | Time-to-kill experiments | Extract was able to kill >90% of all cells of Candida albicans at a concentration of 1.9 mg/mL after a 10 hour incubation | [22] |
Antifungal | Ethanol | Bark | Serial broth microdilution | Extracts exhibited activities with MIC values of 3.1 mg/mL against Candida albicans | [46] |
Anti-HIV | Aqueous | Root | RNA-dependent-DNA polymerase (RDDP) activity of HIV-1 reverse transcriptase | Extracts exhibited activities with half maximal inhibitory concentration (IC50) value of 80.0 µg/mL | [44] |
Anti-HIV | Methanol | Root | RNA-dependent-DNA polymerase (RDDP) activity of HIV-1 reverse transcriptase | Extracts exhibited activities with IC50 value of 131.0 µg/mL | [44] |
Anti-HIV | Aqueous | Root | RNase H assay | Extracts exhibited activities with IC50 value of 31.2 µg/mL | [44] |
Anti-HIV | Methanol | Root | RNase H assay | Extracts exhibited activities with IC50 value of 30.0 µg/mL | [44] |
Anti-HIV | 70% acetone | Stem bark | NF-ĸB assay | Extracts showed inhibitory activities of 45% to 54% | [50] |
Anti-HIV | Chloroform | Stem bark | NF-ĸB assay | Extracts showed inhibitory activities of 57% to 73% | [50] |
Anti-HIV | Ethyl acetate | Stem bark | NF-ĸB assay | Extracts showed inhibitory activities of 72% to 76% | [50] |
Anti-HIV | 70% acetone | Stem bark | HeLa-Tat-Luc assay | Extracts showed inhibitory activities of 22% to 43% | [50] |
Anti-HIV | Chloroform | Stem bark | HeLa-Tat-Luc assay | Extracts showed inhibitory activities of 28% to 76% | [50] |
Anti-HIV | Chloroform | Stem bark | HeLa-Tat-Luc assay | Extracts showed inhibitory activities of 63% to 75% | [50] |
Anti-HIV | Methanol | Bark | Anti-HIV-1iiiB assay | Exhibited activities with half maximal effective concentration (EC50) value of 0.1µg/mL and 0.2µg/mL | [62] |
Anti-HIV | Methanol | Bark | Anti-HIV-1iiiB assay | Exhibited activities with EC50 value of 3.5 µg/mL | [23] |
Anti-HIV | Ethanol | Bark | HIV-RT colorimetric assay | Extract exhibited inhibitory activity of 20% | [46] |
Anti-HIV | Aqueous | Bark | Reverse transcriptase (RT) assay | Extract showed inhibition ranging from 25% to 40% | [63] |
Anti-inflammatory | Aqueous | Root bark | Cyclooxygenase (COX) inhibition assay | Extract exhibited 90% PGE2 inhibition in lipopolysaccharide (LPS) induced RAW 264.7 macrophages | [64] |
Anti-inflammatory | Ethanol | Bark | Lipoxygenase (15-LOX) inhibitory assay | Extract exhibited activities with IC50 value of 80.2 µg/mL | [46] |
Antioxidant | Aqueous | Roots | 2,2-dipheny-l-picrylhydrazyl (DPPH) free radical scavenging assay | Above 200 µg/mL, the extract showed 80% scavenging activity | [65] |
Antioxidant | Ethanol | Roots | DPPH free radical scavenging assay | Above 100 µg/mL, the extract showed 80% scavenging activity | [65] |
Antioxidant | Aqueous | Roots | DPPH free radical scavenging assay | Above 200 µg/mL, the extract showed 80% scavenging activity | [39] |
Antioxidant | Ethanol | Roots | DPPH free radical scavenging assay | Above 100 µg/mL, the extract showed 80% scavenging activity | [39] |
Antioxidant | Methanol | Bark | Hydroxyl (•OH) radical scavenging assay | Exhibited activities with IC50 values of 3.6 mg/mL | [48] |
Antioxidant | Methanol | Bark | Super oxide (SO) assay | Exhibited activities with IC50 values of 1.6 mg/mL | [48] |
Antioxidant | Methanol | Bark | Nitric oxide (NO) radical scavenging assay | Exhibited activities with IC50 values of 3.6 mg/mL | [48] |
Antioxidant | Methanol | Bark | Iron chelating property assay | Exhibited activities with IC50 values of 3.9 mg/mL | [48] |
Antioxidant | Methanol | Bark | DPPH free radical scavenging assay | Exhibited activities with IC50 values of 0.7 mg/mL | [48] |
Antioxidant | Methanol | Bark | 2,2´-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays | Exhibited activities with IC50 values of 4.1 mg/mL | [48] |
Antioxidant | Methanol | Leaves | DPPH free radical scavenging assay | Exhibited activities with EC50 value of 2.8 mg/mL | [57] |
Antiplasmodial | Dichloromethane | Bark | Plasmodium falciparum Plasmodium falciparum lactate dehydrogenase assay | Extract exhibited activities with IC50 value of 5.1 µg/mL | [48] |
Anti-protozoan | Aqueous | Bark | Serial two-fold dilution | Extract exhibited activities with MIC value of 9.7 mg/mL against Trichomonas vaginalis | [66] |
Anti-pyretic | Methanol | Bark | In vivo experiments using female and male Sprague-Dawley rats | Extracts exhibited potential to reduce pyrexia in the induced rats and activities were time and concentration dependent with extracts showing activity as early as from 30 minutes and even at the lowest concentration of 100 mg/kg | [48] |
Hypoglycaemic | Acetone | Stem bark | In vitro anti-diabetic and toxicity screening against murine C2C12 myoblasts, Chang liver cells and 3T3-L1 preadipocytes | Extracts had potential of 138.6% to lower blood glucose levels at a concentration of 50 µg/mL against 3T3-L1 preadipocytes and 100% against both C2C12 myoblasts and Chang liver cells. | [67] |
Hypoglycaemic | Acetone | Stem bark | α-amylase inhibiting activity | Extract exhibited activity with IC50 value of 1.1 µg/mL | [67] |
Hypoglycaemic | Acetone | Stem bark | α-glucosidase inhibiting activity | Extract exhibited activity with IC50 value of 50.6 µg/mL | [67] |
Larvicidal | Dichloromethane | Bark | Larvicidal assay on Culex quinquefascitus larvae | Extracts exhibited activities with 60% mortality and IC50 value of 18.2 µg/mL | [48] |
Larvicidal | Methanol | Bark | Larvicidal assay on Culex quinquefascitus larvae | Extracts exhibited activities with 47% mortality and IC50 value of 9.8 µg/mL | [48] |
Cytotoxicity | Ethanol | Stem bark | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) calorimetric assay | Extracts exhibited activities at 12.5 µg/mL showing 90% and 40% of viable 3T3-L1 preadipocytes and Chang liver cells, respectively of the control | [67] |
Cytotoxicity | Ethanol | Stem bark | XTT (sodium 3’-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate) colorimetric assay | Extracts exhibited activities with IC50 values >100.0 µg/mL in both Vero cells and MCF-7 cell line | [53] |
Cytotoxicity | Methanol | Stem bark | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay | Extracts exhibited activities with half maximal cytotoxic concentration (CC50) value of 3.7 mg/mL | [62] |
Cytotoxicity | Dichloromethane | Bark | MTT cell proliferation assay | Extracts exhibited activities with the median lethal concentration (LC50) value of 512.0 µg/mL and 394.0 µg/mL against human embryonic kidney (HEK293) and human hepatocellular carcinoma (HepG2) cells, respectively | [48] |
Cytotoxicity | Methanol | Bark | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) calorimetric assay | Extract exhibited activities with CC50 value of 200.0 µg/mL and selective index (SI) value of 57.1 | [23] |
Cytotoxicity | Aqueous | Bark | MTT cell proliferation assay | Extracts exhibited activities in all the three human tumour cancer cell lines | [63] |
Cytotoxicity | 70% acetone | Stem bark | Cytotoxicity assay on MT2 cells | Extracts showed cell death of 22.7% after 36 h at the highest concentration tested of 15 µg/mL | [50] |
Cytotoxicity | Chloroform | Stem bark | Cytotoxicity assay on MT2 cells | Extracts showed cell death of 27.6% after 36 h at the highest concentration tested of 15 µg/mL | [50] |
Cytotoxicity | Ethyl acetate | Stem bark | Cytotoxicity assay on MT2 cells | Extracts showed cell death of 17.1% after 36 h at the highest concentration tested of 15 µg/mL | [50] |
Antimutagenicity | Methanol | Leaves | Ames test | Extract exhibited weak antimutagenic activities with 23.2% inhibition of 4-nitroquinoline 1-oxide in Salmonella typhimurium TA98 and 21.3% in strain TA100 at the assayed concentration of 5 mg/mL | [57] |
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Maroyi, A.; Semenya, S.S. Medicinal Uses, Phytochemistry and Pharmacological Properties of Elaeodendron transvaalense. Nutrients 2019, 11, 545. https://doi.org/10.3390/nu11030545
Maroyi A, Semenya SS. Medicinal Uses, Phytochemistry and Pharmacological Properties of Elaeodendron transvaalense. Nutrients. 2019; 11(3):545. https://doi.org/10.3390/nu11030545
Chicago/Turabian StyleMaroyi, Alfred, and Sebua Silas Semenya. 2019. "Medicinal Uses, Phytochemistry and Pharmacological Properties of Elaeodendron transvaalense" Nutrients 11, no. 3: 545. https://doi.org/10.3390/nu11030545