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Optimizing water use
efficiency in C4 crops
Daniel Carvalho
PhD. Candidate in Agronomy and Horticulture
Advisor: James C. Schnable
Why am I here?
Carrão et al, 2016
Drought risk
Background
• C4 photosynthesis is more
efficient and has better water
use efficiency (WUE)
http://evolution.berkeley.edu/evolibrary/images/interviews/stoma_diagram.gif
• Agriculture increases strain in world
water use
Background
• C3 and C4 plants have different WUE
• Questions:
- Are there differences across C4
plants?
- Can we use them to reduce the
strain in water resources?
http://msir.co/files/2012/08/T-L_Pivot_Line.jpg
Methods and facilities used
LI-COR 6800 photosynthesis measurements
Photo:EvanLaBrant
Lemnatec at NIC Greenhouse
Comparative genomics Comparative phenomics
Sorghum
Maize
Dichanthelium
Photosynthetic Water Use Efficiency
Photos: Schnable Lab
1
2
3
4
5
Future steps
• Evaluating the relationships between water use efficiency of LI-COR
and imaging data
Sorghum Tef
Thank You
waterforfood.nebraska.edu
Prof. James Schnable Dr. Sunil Kumar
@SchnableLab
@deCarvalhoD90
WUE by C4 subtype
Species C4 subtype
Dichanthelium oligosanthes C3
Panicum miliaceum NAD-ME
Eleusine coracana NAD-ME
Zea mays NADP-ME
Sorghum bicolor NADP-ME
Coix lacryma-jobi NADP-ME
Pennisetum glaucum NADP-ME
Setaria italica NADP-ME
Echinochloa esculenta NADP-ME
Echinochloa colona NADP-ME
Danthoniopsis dinteri NADP-ME
Urochloa fusca PCK
Eragrostis tef PCK

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1) Daniel Carvalho

  • 1. Optimizing water use efficiency in C4 crops Daniel Carvalho PhD. Candidate in Agronomy and Horticulture Advisor: James C. Schnable
  • 2. Why am I here? Carrão et al, 2016 Drought risk
  • 3. Background • C4 photosynthesis is more efficient and has better water use efficiency (WUE) http://evolution.berkeley.edu/evolibrary/images/interviews/stoma_diagram.gif • Agriculture increases strain in world water use
  • 4. Background • C3 and C4 plants have different WUE • Questions: - Are there differences across C4 plants? - Can we use them to reduce the strain in water resources? http://msir.co/files/2012/08/T-L_Pivot_Line.jpg
  • 5. Methods and facilities used LI-COR 6800 photosynthesis measurements Photo:EvanLaBrant Lemnatec at NIC Greenhouse Comparative genomics Comparative phenomics Sorghum Maize Dichanthelium
  • 6. Photosynthetic Water Use Efficiency Photos: Schnable Lab 1 2 3 4 5
  • 7. Future steps • Evaluating the relationships between water use efficiency of LI-COR and imaging data Sorghum Tef
  • 8. Thank You waterforfood.nebraska.edu Prof. James Schnable Dr. Sunil Kumar @SchnableLab @deCarvalhoD90
  • 9. WUE by C4 subtype Species C4 subtype Dichanthelium oligosanthes C3 Panicum miliaceum NAD-ME Eleusine coracana NAD-ME Zea mays NADP-ME Sorghum bicolor NADP-ME Coix lacryma-jobi NADP-ME Pennisetum glaucum NADP-ME Setaria italica NADP-ME Echinochloa esculenta NADP-ME Echinochloa colona NADP-ME Danthoniopsis dinteri NADP-ME Urochloa fusca PCK Eragrostis tef PCK

Editor's Notes

  1. Northeast-Brazil is in the Drought polygon (term created by the government due to recurrent dry periods).
  2. Water loss through transpiration Different plants different degrees of transpiration, different types of photosynthesis Are there big differences across different C4 plants?
  3. Brazilian government has allowed me to work on my phd project with genomics data, what w4f allowed me was the phenomics part. Left: FIX FIGURE ADD EXTRA SPP AND FIGURE. CHANGE GEVO CONFIG TO MAKE THINGS THICKER. Gevo (Figure Gevo panel with pictures of plants) ] Right: Phenomics figure LICOR instataneous photosynthetic water use efficiency, Lemnatec long term functional (photosynthetic?) WUE, agronomic WUE (biomass)
  4. FIX: Add a tree in the slide, make sure to add C3 groups to each origin WUE differs a lot across different origins, I’m optimistic that I’m able we can learn things to make C4 crops more WUE
  5. Still working on evaluating relationship between the WUE of LICOR and Lemnatec data Remind how their different from each other
  6. This work would not be possible without the crops domesticated by different cultures all around the world, which we have access to. Questions: Lowering infrastructure costs for water irrigation Genetic engineering acceptance by other countries (overcome limitations like drought and food security) * Industry control of food plants (maybe...) ** They don’t like Monsanto and having companies controling what they eat....
  7. Still working on evaluating relationship between the WUE of LICOR and Lemnatec data Remind how their different from each other